日本血吸虫感染小鼠IL17IL23动态变化研究

Dynamic expressions of IL-17 and IL-23 in mice infected with Schistosoma japonicum

目的观察日本血吸虫感染小鼠IL 17A、IL 23p19的表达,探讨IL 17、IL 23在免疫调控中的作用。方法建立日本血吸虫感染的小鼠模型,采用ELISA和RT PCR法检测小鼠感染后1、2、4、6、8及10周脾细胞培养液上清中IL 17A、IL 23p19蛋白水平和mRNA水平的表达。结果蛋白及mRNA表达结果显示,感染小鼠IL 17A与IL 23p19的变化趋势基本一致。感染小鼠IL 17A蛋白表达水平于感染后1周迅速升高,之后持续下降,8周时表达水平低于正常对照组;其mRNA表达也于感染后1周升高,第2周开始下降。而IL 23p19蛋白的表达水平于感染后1周升高,第2周到达高峰,第4周开始下降;其mRNA水平起先一直在正常范围波动,感染后4周开始升高,第6周达到峰值,之后逐渐下降。结论 IL 17和IL 23在日本血吸虫感染小鼠体内协同表达,并于感染早期呈现显著上升趋势,提示IL 17、IL 23参与了血吸虫感染极早期的免疫病理过程。

Objective To investigate the dynamic expressions of IL-17 and IL-23 in mice with Schistosoma japonicum infection.Method A murine model of the infection of S.japonicum was established.The suspension of spleen cells incubated with ConA was collected at 0,1,2,4,6,8 and 10 weeks post-infection.Sandwich ELISA and RT-PCR were used to measure the expressions of IL-17A and IL-23p19 on protein and mRNA level.Results The dynamic changes of IL-17A and IL-23p19 showed a positive correlation.The level of IL-17A increased and reached the peak at 1 week after the infection,reduced at 4 weeks after the infection,and was even lower at 8 weeks post-infection.The level of IL-17 mRNA increased at 1 week post-infection,and then decreased gradually at 2 weeks post-infection.Being consistent with the dynamic expression of IL-17A,the IL-23p19 expression increased at 1 week post-infection,went up to the peak at 2 weeks post-infection,and gradually reduced into the normal level at 4 weeks.However,the expression of IL-23p19 mRNA fluctuated in the normal range,increased at 4 weeks post-infection,and reached the peak at 6 weeks post-infection.Conclusions IL-17 and IL-23 are of co-expression in the mice after schistosome infection.There is a significant increase in the early stage of the infection.IL-17 and IL-23 may play important roles during the immune process in the very early stage of Schistosoma infection.