染色体3p14.2区域一个与鼻咽癌呈负相关EST的鉴定

Identification of an EST down-regulated in nasopharyngeal carcinoma at chromosome 3p14.2

目的 :寻找染色体 3 p14 .2区域与鼻咽癌相关的表达序列标记 (expressedsequencetag ,EST) ,为筛选鼻咽癌候选基因打下基础。方法 :运用生物信息学同源性比较筛选EST的策略 ,结合Northern杂交和逆转录PCR方法 ,检测 3 p14 .2区域的相关ESTs在鼻咽癌和正常鼻咽组织中的表达。结果 :在 3 p14 .2区域筛选到一个在鼻咽癌中低表达的ESTw2 3 3 12 ,与正常鼻咽上皮组织相比较 ,在鼻咽癌细胞株及 13例鼻咽癌活检组织中的 5例其表达下调 (P <0 .0 1)。结论 :染色体 3p14 .2区域ESTw2 3 3 12在鼻咽癌中表达下调 ,提示其可能参与鼻咽癌癌变过程 。

Objective: To identify the expressed sequence tags (EST) associated with nasopharyngeal carcinoma (NPC) at chromosome 3p14.2 for cloning of candidate gene(s) at the loci. Methods: Using ESTs homology analysis in bioinformatics combined with Northern blots and reverse transcription-PCR, the expression of relative ESTs at 3p14.2 was detected in nasopharyngeal carcinoma tissues in comparison with normal nasopharyngeal epithelia. Results: Comparing with expression of normal nasopharyngeal epithelia, EST w23312 was down-regulation in nasopharyngeal carcinoma cell line and five of thirteen nasopharyngeal biopsies( P < 0.01). Conclusion: W23312 is down-regulation in NPC. It may play a role in NPC carcinogenesis and provide a new clue in cloning of NPC associated gene(s).