胃癌及癌前病变组织中MUC5AC基因的异常表达及意义

Abberant expression of MUC5AC gene in the tissues of precancerous lesion and gastric cancer

目的 :揭示正常胃粘膜、癌前病变和胃癌组织中MUC5AC基因的表达与其临床病理分型之间的联系。方法 :应用免疫组织化学SP法检测人正常胃粘膜、癌前病变粘膜以及胃癌组织中MUC5AC核粘蛋白的表达。结果 :人正常胃粘膜中的浅表 1/ 3范围内广泛分布MUC5AC基因产物 ( 10 0 % ) ,肠化、不典型增生和胃癌组织中的表达阳性率分别是 2 9.6%、10 0 %和 4 0 .0 %。胃癌组织中MUC5AC核粘蛋白的表达与胃癌患者的性别、肿瘤的部位和大小、淋巴结转移、肿瘤的分化和浸润、临床分期无关 (P >0 .0 5 ) ,但MUC5AC阳性组与阴性组之间的胃癌的Lau ren’氏分型差别显著 (P <0 .0 5 )。结论 :MUC5AC是组成胃粘膜的粘蛋白基因之一 ,在胃癌和癌前病变是下调表达 。

Objectives:To determine MUC5AC gene expression in the tissues of normal gastric mucosa, precancerous lesion and gastric cancer, and to elucidate the association of abberant expression of MUC5AC gene in gastric cancer with clinicopathological factors. Method: MUC5AC apomucin was detected by immunohistochemical assay. Results: MUC5AC apomucin was distributed in the 1/3 superjacent gastric mucosa (100%). The detective rates in the tissues of intestinal metaplasia,atypical hyperplasia and gastric cancer were 29.6%,100%and 40.0%, respectively. No association was found between the expression of MUC5AC apomucin in the neoplastic gastric epithelium and patients' sexes, locations and size of cancerous tissues,metastasis of lymph nodes, differentiations, invasiveness or clinical stages ( P >0.05). However,the Lauren's types was significantly different ( P < 0.05)between MUC5AC positive group and MUC5AC negative group. Conclusions:MUC5AC gene is one of the mucin gene patterns in the gastric mucosa, and its expression is notably decreased in the precancerous lesion and gastric cancer. MUC5AC apomucin expression is associated with Lauren's type of gastric cancer.