肺癌异位HCG和环核苷酸的检测及其意义探讨

A study on the ectopic HCG and cyclic nucliotides in patients with primary lung cancer

目的 :探讨肿瘤性异位HCG的作用机理。方法 :应用放射免疫分析 (RIA)方法检测 3 0例原发性肺癌患者血浆及癌组织悬液中人绒毛膜促性腺激素 (HCG和 β HCG)和环核苷酸的含量 .结果 :肺癌患者血清HCG和β HCG含量在术前组明显高于正常对照组 (P <0 .0 1) ,而术后组较术前组显著降低 (P <0 .0 0 1)。癌组织悬液中HCG和 β HCG明显高于肿瘤远端正常肺组织 (P <0 .0 0 1)。同时检测部分患者血浆和癌组织中环核苷酸含量的结果显示肺癌患者血浆环鸟苷酸 (cGMP)显著高于良性肺部疾病组 ,手术后cGMP显著降低 ,而在癌组织悬液中环腺苷酸 (cAMP)显著低于正常组织组 ,总的趋势是血浆和癌组织中cAMP/cGMP比率明显降低 (P <0 .0 5 )。结论 :本文结果提示肿瘤性异位HCG作为一种自分泌因子 ,可能通过环核苷酸这一激素效应的中间环节 ,在肿瘤细胞自身调节生长、分化。

Objective:To explore the effect of ectopic human chorionic gonadotropin (HCG) from lung cancer. Methods:HCG and its beta subunit (β HCG) in the plasma and tumor tissue of 30 primary lung cancer patients were determined by radioimmunoassay(RIA).In some of the cases, plasma and tumortissue cyclic nucleotides were also measured. Results:The preoperative serum HCG /β HCG levels of the patients were much higher than that of the control group ( P < 0.001 ), while the postoperative HCG/β HCG levels were dropped markedly ( P < 0.001).The HCG /β HCG in the tumor tissue homogenate were strikingly higher than that in the surrounding normal lung tissue ( P < 0.001). The plasma cGMP level was significantly higher in the patient with lung cancer than that with benign lung disease. However,the cAMP level in the tissue from lung cancer was significenotly lower. The general tendency was that cAMP / cGMP radio of the plasma and tumor tissue of the patient decreased. Conclusion:Our results suggest that the ectopic HCG from tumor tissue, as an autocrine cytokine, may through cyclic nucleotides, the mediator or second messenger of hormones affect the growth, differentiation and biological behavior of tumor cells.