SD大鼠2型糖尿病动物模型的建立及胰腺组织SUR1 mRNA的表达

Establishment of Type 2 Diabetes Mellitus Animal Model of SD Rats and Expression of Pancreatic Tissue SUR1 mRNA

[目的]链脲佐菌素(streptozotocin,STZ)结合高糖高脂饮食诱导建立2型糖尿病大鼠模型,检测体重、血糖、胰岛素、胰岛素敏感指数(ISI)、胰腺组织SUR1 mRNA表达的变化。[方法]30只健康雄性SD大鼠随机分为模型组(20只)、空白组(10只)。模型组喂饲高糖高脂饲料4周后,用STZ 30mg/(kg·bw)一次性左下腹腔注射。检测注射STZ后第1周、第4周、第8周、第12周空腹体重、血糖、胰岛素、胰岛素敏感指数等指标,进行统计分析。剖杀大鼠,取胰腺,RT-PCR方法检测胰腺组织SUR1 mRNA的表达。[结果]动物成模率为75%。注射STZ后1、4、8、12周,模型组血糖值均明显升高,与空白组比较,P<0.01;ISI均明显降低,与空白组比较,P<0.01。模型组SUR1 mRNA表达显著低于对照组(P<0.05)。[结论]STZ一次性左下腹腔注射结合高糖高脂饮食可成功诱导2型糖尿病大鼠模型。SUR1 mRNA的降低可能是糖尿病发病的分子机制之一。

[Objective] The research aimed to establish a rat model for type 2 diabetes mellitus（T2DM）induced by streptozotocin（STZ）in combination with high-sugar and high-fat diet and detect the changes of weight,glucose,insulin,insulin sensitivity index, mRNA expression of pancreatic tissue SUR1. [Method] 30 healthy male SD rats were randomly divided into model group（20 rats ）and blank group（10 rats）. After rats in model group was fed with high-sucrose and high-fat diet for 4 weeks, 30 mg STZ was injected by left- lower abdominal cavity injection per one kilogram body weight. The fasting body weight, blood glucose, insulin, insulin sensitivity index（ISI） were detected on the 1st,4th,8th,12th week after injecting STZ for statistical analysis. After rats were killed, pancreas was picked and the expression of pancreatic tissue SUR1 mRNA by using RT-PCR method. [Result] The success rate of T2DM rat model was 75%. On the 1st, 4th, 8th and 12th week after STZ injection, blood glucose in model group significantly increased than blank group（P0.01）. ISI in model group significantly decreased than blank group （P0.01）. The expression of SUR1 mRNA in model group was significantly lower than blank group（P0.05）. [Conclusion] T2DM animal model could be induced by high-sugar and high-fat diet and STZ left- lower abdominal cavity injection. The decrease of SUR1 mRNA might be one of molecular pathogenesis of diabetes.