复合微生态制剂与饲用抗生素对肉鸡血清生化指标和肠道酶活性的影响

Effects of Compound Probiotics and Feed Antibiotics on the Serum Biochemical Indices and Intestinal Enzyme Activity of Broilers

研究添加复合微生态制剂和饲用抗生素对肉鸡血清生化指标和肠道酶活性的影响。试验选取500只1日龄雌性健康AA肉鸡随机分为5组,每组4个重复,每个重复25只。试验预试期7d,Ⅰ组为对照组,饲喂不含抗生素的基础饲粮,Ⅴ组为饲用抗生素组,Ⅱ、Ⅲ、Ⅳ组为微生态制剂组,添加量分别为1.0‰、2.0‰、4.0‰。正试期为35d。结果表明,除42日龄外,其余各试验阶段Ⅲ组肉鸡血清总蛋白含量均显著高于对照组和抗生素组(P<0.05);除14日龄外,Ⅲ组肉鸡血清尿素氮含量均显著低于对照组和抗生素组(P<0.05);在每个试验阶段,Ⅲ组回肠内容物蛋白酶活性均显著高于对照组和抗生素组(P<0.05),且除14日龄外,Ⅲ组肉鸡空肠内容物蛋白酶活性最高,显著高于对照组和抗生素组(P<0.05);除14日龄外,Ⅲ组空肠和回肠内容物淀粉酶活性均最高,显著高于对照组和抗生素组(P<0.05)。由此可知,2.0‰复合微生态制剂能够显著提高肉鸡血清总蛋白水平,增强空肠和回肠内容物蛋白酶和淀粉酶活性,同时在一定程度上降低了肉鸡血清尿素氮含量。

The effects of adding compound micro-ecological preparation and feed antibiotics on the serum biochemical indices and intestinal enzyme activity of broilers were studied. Five hundred one-day-old female AA broiler chicken were randomly divided into five groups with 4 repetitions in each group and 25 chickens per repetition. The pre-trail period of the experiment was 7 days. The chicken in the control group（groupⅠ）were only fed with the basal diet（no antibiotics）,chicken in groupⅤwas fed with antibiotics and chicken in group Ⅱ,Ⅲ and Ⅳ were fed with feeding the diet with adding 1.0‰,2.0‰ and 4.0‰ micro-ecological preparation. The test period lasted for 35 days. The results showed that the content of serum total protein in group Ⅲ in each test stages was significantly higher than that in control group and antibiotics group（P0.05）, except 42-day-old. Except 14- day-old, the content of serum urea nitrogen in group Ⅲ of broilers was significantly lower than that in control group and antibiotics group （P0.05）. In all test stages, the activity of protease in ileum contents in group Ⅲ was significantly higher than that in control group and antibiotics group （P0.05）. And the activity of protease in jejunum contents in group Ⅲ was significantly higher than that in control group and antibiotics group （P0.05）, except 14-day-old. Except 14-day-old, the activity of amylase in ileum and jejunum contents in group Ⅲ was significantly higher than that in control group and antibiotics group（P0.05）. Therefore, 2.0‰ compound micro-ecological preparation could significantly increase the level of total protein in the serum of broilers, enhance the activities of protease and amylase in ileum and jejunum contents and decrease the content of serum urea nitrogen of broilers to some degree.