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丹酚酸B预处理EPCs对AMI大鼠移植骨髓间充质干细胞后心肌微环境的影响 被引量:7

Effect of EPCs pretreated with salvianolic acid B in microenvironment of myocardial muscle after transplantation of bone mesenchymal stem cells in AMI rats
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摘要 [目的]探讨中药丹酚酸B预处理的内皮祖细胞(EPCs)对急性心肌梗死(AMI)大鼠移植骨髓间充质干细胞(BMSCs)后心肌微环境的影响。[方法]56只AM I模型大鼠随机分为假手术组、模型组、BM SCs组、EPCs+BM SCs组,其中治疗组用丹酚酸B最佳药物浓度处理的EPCs与BM SCs混合,在大鼠AM I区周边分5点注射。免疫荧光和免疫组化方法检测AM I大鼠梗死区心肌收缩蛋白α型肌球蛋白重链(α-M H C)和I型胶原蛋白的表达。[结果]细胞移植4周后,各细胞联合移植组中,2∶14、∶1和8∶1组在Brdu集中表达区域均有不同强弱程度的α-MHC阳性表达,除假手术组较少外,所有接受冠状动脉结扎的大鼠心肌梗死区I型胶原表达均升高。[结论]BMSCs在丹酚酸B预处理的EPCs干预的心肌微环境下可向心肌细胞分化,可预防或改善心室重塑的发生。 [Objective] To investigate the effect of salvianolic acid B preconditioned endothelial progenitor cells(EPCs) combined with implantation of bone mesenchymal stem cells(BMSCs) on the gene expression in rats with acute myocardial infarction(AMI).[Methods] Forty-two AMI rats were divided randomly into sham operation model,BMSCs and EPCs combined with BMSCs transplantation group,and EPCs preconditioned with salvianolic acid B combined with transplantation of BMSCs were injected into five points of heart ischemia area.[Result] After 4 weeks,among each combined cell transplantation group,in 2∶1,4∶1 and 8∶1 group in the area of Brdu concentrated expression positive α-MHC expression at different degree could be found.Except that the sham operation group had less expression,all the area with myocardial infarction underwent coronary artery ligation in rats the type I collagen expression increased.[Conclusion] Salvianolic acid B-preconditioned EPCs with transplanted BMSCs under the microenvironment of myocardial muscle can be differentiated into myocardial cells.They can prevent or improve the occurrence of ventricular remodeling.
出处 《天津中医药》 CAS 2012年第2期117-120,共4页 Tianjin Journal of Traditional Chinese Medicine
基金 天津市应用基础及前沿技术研究计划(09JCYBJC12200) 教育部高等学校博士学科点专项科研基金项目(20111210120010 20101210110004)
关键词 丹酚酸B 急性心肌梗死 内皮祖细胞 骨髓间充质干细胞 细胞移植 Salvianolic acid B(SalB) acute myocardialin infarction(AMI) endothelial progenitor cells(EPCs) bone mesenchymal stem cells(BMSCs) cell transplantation
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  • 1朱丽华,江洪,赵冬冬.犬骨髓血管内皮祖细胞的分离和诱导分化[J].微循环学杂志,2005,15(1):21-23. 被引量:1
  • 2刘立琼,李云霞.大鼠左室肌凝蛋白重链同功酶谱的增龄性变化[J].中国应用生理学杂志,1994,10(1):1-4. 被引量:1
  • 3Murayama T,Tepper OM,Silver M,et al. Determination of bone marrow- derived endothelial progenitor cell significance in angiogenic growth factorinduced neovascularization in vivo. [J] Exp Hematol, 2002,30(8):967-972.
  • 4Makino S,Fukuda K,Miyoshi S,et at.Cardiomyocytes can be generated from marrowstromal cells in vitro [J].JClinical Invest,1999,103:687- 705.
  • 5Mangi, AA. Mesenchymal stem cells modified with Akt prevent remodeling and restore performance of infracted hearts [J]. Nat Med, 2003,9(9): 1195 - 1201.
  • 6Asahara T,Murohara T, Alison S,et al. Isolation of putative progenitor endothelial cells of angiogenesis.[J] Science,1997275(5302).964-967.
  • 7Peichev M,Naiyer AJ,Pereira D,et al. Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors[J]. Blood, 2000,95:952-958.
  • 8Kawamoto A, Gwon HC, Iwaguro H, et al. Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial schemia[J]. Circulation,2001,103(5): 634-637.
  • 9Murayama T,Tepper OM,Silver M,et al. Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo [J]. Exp Hematol, 2002,30(8):967-972.
  • 10Reyes M, Dudek A, Jahagirdar B, et al. Origin of endothelial Progenitors in human postnatal bone marrow [J]. J Clin Invest,2002,109: 337-346.

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