熄风胶囊对氯化锂-匹罗卡品癫痫大鼠皮质和海马多药耐药相关蛋白1表达影响的研究

Effect of Xi Feng capsule on expression of MRP1 of hippocampus and cortex in epileptic rats induced by lithium-pilocarpine

[目的]研究熄风胶囊对氯化锂-匹罗卡品癫痫大鼠皮质和海马多药耐药相关蛋白1(MRP1)表达的影响。[方法]1)利用氯化锂-匹罗卡品制作癫痫大鼠模型。2)实验大鼠按随机数字表法随机分为正常组、模型组、熄风胶囊高剂量组(熄高组)、熄风胶囊中剂量组(熄中组)、熄风胶囊低剂量组(熄低组)、熄风胶囊大剂量+卡马西平(CBZ)组(联高组)、熄风胶囊大剂量+CBZ1/2剂量(联低组)和CBZ组。3)通过Western-blot方法检测癫痫大鼠海马和皮质MRP1的表达程度。[结果]实验结果显示治疗组与模型组之间比较差异有统计学意义(P<0.05)。[结论]熄风胶囊能有效抑制MRP1的过度表达。

[Objective] To study the intervention effect of Xi Feng capsule on medicine-related protein 1（MRP1） of hippocampus and cortex in epileptic rats induced by Lithium-Pilocarpine.[Methods] 1）Epilepsy was duplicated in rats with lithium-pilocarpine chemical ignition.2）All the experimental rats were randomly divided into eight groups： normal control group,model group,Xi Feng intervention group（Xi Feng high-dose group）,Xi Feng intervention group（Xi Feng middle-dose group）,Xi Feng intervention group（Xi Feng low-dose group）,Xi Feng high-dose＋CBZ group,Xi Feng high-dose＋CBZ 1/2 dose group,CBZ group.3）The expression of MRP1 of hippocampus and cortex in epileptic rats was observed by Western-blot.[Results] The result of Western-blot method suggested that there was a significant difference between intervention group of Xi Feng capsule and model group（P0.05）.[Conclusion] Xi Feng capsule single and combined with CBZ could efficiently depress the overexpression of MRP1 of the hippocampus and cortex in epileptic rats induced by Lithium-Pilocarpine.