子宫平滑肌和胎膜组织表皮生长因子受体mRNA表达水平与分娩发动的关系

Correlation between Epidermal Growth Factor Receptor Messenger RNA Expression and the Onset of Labor

目的 探讨子宫平滑肌和胎膜组织表皮生长因子受体 (EGFR)mRNA表达水平与分娩发动的关系。方法 用半定量逆转录聚合酶链反应检测分娩发动前、后各 10例产妇子宫平滑肌和胎膜组织的EGFRmRNA表达水平。结果 子宫平滑肌EGFRmRNA表达水平由分娩发动前的 0 .91± 0 .3 2增高至分娩发动后的 1.3 3± 0 .2 3 ,两者比较 ,差异有显著性 (P <0 .0 5 ) ;胎膜组织EGFRmRNA表达水平由分娩发动前的 1.0 5± 0 .3 0增高至分娩发动后的 1.65± 0 .71,两者比较 ,差异有显著性 (P<0 .0 5 )。结论 分娩发动前、后子宫平滑肌及胎膜组织中EGFRmRNA表达水平升高 ;并与分娩发动有密切关系 ,是诱发子宫平滑肌收缩的重要因素。

Objective To study the correlation between epidermal growth factor receptor messenger RNA expression and the onset of labor. Method Semiquantitative reverse transcriptase polymerase chain reaction,using GAPDH as internal standard,was applied to determine the levels of epidermal growth factor receptor messenger RNA in human myometrium ( n =20) and fetal membranes ( n =20) before and during labor. Results In myometrium,epidermal growth factor receptor messenger RNA increased from 0.91±0.32 before labor to 1.33±0.23 after the onset of labor ( P <0.05).In fetal membranes, epidermal growth factor receptor messenger RNA increased from 1.05±0.30 before labor to 1.65±0.71 after the onset of labor ( P <0.05). Conclusion The study indicates it is Up regulation of epidermal growth factor receptors messenger RNA expression in myometrium and fetal membrane before and after the onset of labor.