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富血小板纤维蛋白(PRF)诱导骨髓间质干细胞分化成骨细胞的实验研究 被引量：2

The study of the function of PRF in the process of inducing MSCs into osteoblasts
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摘要 目的研究富血小板纤维蛋白在诱导骨髓间质干细胞向成骨细胞转化的作用。方法取7天新西兰大白兔股骨,分离骨髓间质干细胞进行体外培养,在各自的培养条件下分为三组:实验组(不同剂量的PRF组)、空白对照组(MSCS组)和阳性对照组(BMP-2组)。三组细胞的比较采用MTT测定细胞增值率、PNPP法测定碱性磷酸酶的表达、免疫组化标记Ⅰ型胶原蛋白的表达、检测细胞为成骨细胞。结果细胞形态学观察,MSCS分化后,细胞形态从长梭形变成三角形,多角形,立方形;MTT测定细胞增殖显示,随着PRF膜剂量的增加,细胞增殖数量增加,PRF剂量为4,5mL时,与BMP-2组差别不大;ALP活性结果显示,MSCS分化后,ALP活性远高于MSCS细胞。两者差异具有显著统计学意义(t=24.608,p=0.000);免疫组化标记Ⅰ型胶原结果显示,PRF组分化后的MSCSⅠ型胶原表达显著。结论富血小板纤维蛋白可以诱导骨髓间质干细胞分化为成骨细胞,分化的类成骨细胞具有成骨细胞的特性,可以作为自体材料应用于口腔种植学领域里骨缺的修复。 【Objective】 to study the function of PRF in the process of inducing MSCs into osteoblasts.【Methods】 isolate MSCs from the femur of 7-day-old rabbits and divided into 3 groups.Experimental group（different does of PRF）,control group（MSCs） and positive control group（BMP-2）.Use MTT assay to calcuLate cell proliferation rates of each group,use PNPP assay to measure expression of alkaline phosphatase（ALP）,and use IHC to detect collagen one.【Results】 morphological observation shows cells shape change from long spindle into triangle,quadrangle and polygon after MSCs differentiation.MTT shows cell proliferates as PRF does increases.ALP expression of differentiated MSCs is higher than MSCs（t=24.608,p=0.000）.IHC shows collagen one express significantly in experimental group.【Conclusion】 PRF promotes the process of inducing MSCs into osteoblasts.It can be used as autogenous material in bone repairmen of oral implantology.
作者 魏思维 王稚英
机构地区 辽宁医学院附属第二医院口腔颌面外科
出处 《中国医学工程》 2012年第2期17-19,共3页 China Medical Engineering
关键词 富血小板纤维蛋白(PRF) 骨髓间质干细胞 分化 成骨细胞 platelet-rich fibrin PRF mesenchymal stem cell MSCS trans-differentiation osteoblast cells
分类号 R331.143 [医药卫生—人体生理学]
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参考文献14

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共引文献37

同被引文献43

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二级引证文献9

中国医学工程
2012年 第2期

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