脑室注射高渗氯化钠诱导脑内c-fos与egr-1表达以及与AVP神经元的关系

Expression of c-fos and egr-1 in Rat Brain Induced by Intracerebroven tricular Injection of Hypertonic Saline and the Relation between c-fos and Vasopressin Neurons

目的 观察脑室注射高渗氯化钠诱导的脑内ｃ－ｆｏｓ 与ｅｇｒ－１ 的表达特征，并用双重细胞染色方法观察脑内ｃ－ｆｏｓ 表达与下丘脑视上核（ＳＯＮ）中加压素（ＡＶＰ） 能神经元之间的关系。方法 用ＳＤ 种系清醒大鼠，侧脑室微量注射高渗氯化钠，１ｈ 后作ｃ－ｆｏｓ 与ｅｇｒ－ １ 的免疫细胞化学染色和细胞双重染色。结果 （１） 脑室注射高渗氯化钠可在终末血管器官（ＯＶＬＴ）、正中视前核（ ＭＰＮ） 、ＳＯＮ、下丘脑室旁核（ＰＶＮ） 以及后脑的最后区（ＡＰ） 、孤束核（ＳＴＮ） 和臂旁外侧核（ＬＰＢＮ） 中同时诱导ｃ－ｆｏｓ 和ｅｇｒ－ １ 表达，在同一区域内ｅｇｒ－ １ 的表达略低于ｃ－ｆｏｓ，但无统计学差异；（２） 穹窿下器官（ＳＦＯ）中均无ｃ－ｆｏｓ 和ｅｇｒ－１ 表达；（３）ＳＯＮ 中可见部分ＡＶＰ免疫反应阳性与ｃ－ ｆｏｓ表达共存于同一神经元。结论 ｃ－ｆｏｓ 和ｅｇｒ－１ 对脑内高渗刺激诱导的表达部位基本一致，进一步证实了以往的研究结果。另外，脑内注射高渗氯化钠诱导的下丘脑细胞活动至少部分是通过ＡＶＰ能神经元的。

Object The characters of expression of c-fos and egr-1 in the brain provoked by intracerebroventricular (icv) injection of hypertonic saline were observed,and the relation between Fos and AVP-containing neurons in hypothalamus was also studied by double staining.Method C-fos and egr-1 immunohistochemical and double staining were made 1h after icv injection of hypertonic saline in awaking Sprague-Dawley rats.Results Icv injection of 3 mol/L NaCl could induce Fos-like immunoreactive (FLI) and Egr-1-like immunoreactive (ELI) expression in the same areas,such as in organum vasculosum of laminae terminalis (OVLT),median preoptic nucleus (MPN) of forebrain,supraoptic nucleus (SON) and paraventricular nucleus (PVN) of hypothalamus as well as areas postrema (AP),lateral parabrachial nucleus (LPBN) and solitary tract nucleus (STN) of hindbrain.The FLI expression induced by 3 mol/L NaCl was stronger than ELI expression in the same areas,but there was no significant difference.As compared with previous studies,no ELI expression was observed in subfornic organ (SFO).Double staining showed the co-existence of c-fos and vasopressin (AVP)-cells in SON.Conclusion These results suggest that egr-1 expression induced by icv injection of hypertonic saline was in the same areas as c-fos expression in rat brain,and that the response of hypothalamic cells to icv injection of hypertonic saline is partially related to the activation of AVP-containing neurons.