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肾癌中KISS-1通过NF-κB调控MMP-9的表达 被引量：2

KISS-1 regulate the expression of MMP-9 by NF-κB in renal carcinoma
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摘要 目的探讨了NF-κB在KISS-1调控MMP-9的表达中的作用。方法设计并合成了KISS-1基因特异性的siRNA,转染肾癌Caki-1细胞,western blot检测KISS-1、NF-κB和MMP-9蛋白的表达。应用NF-κB抑制剂BAY11-7082处理KISS-1基因表达沉默的Caki-1细胞,western blot检测MMP-9蛋白的表达。结果在Caki-1细胞中,KISS-1基因表达沉默能够显著上调NF-κB蛋白的表达,并显著下调MMP-9蛋白的表达。而BAY 11-7082能够在KISS-1基因表达沉默的Caki-1细胞,显著上调MMP-9蛋白的表达。结论 KISS-1基因在肾癌细胞中能够通过NF-κB调控MMP-9的表达。 [ Objective ] To research the effects of NF - KB on KISS-1 regulate the expression of MMP-9 in re- nal carcinoma. [ Methods] Specific siRNA for KISS-1 gene was designed and synthesized, and then Caki-1 cells were transfected with siRNA - KISS-1 to inhibit the expression of KISS-1 gene. After RNA interference, the ex- pression of KISS-1, NF -kβ and MMP - 9 were detected by western blot. BAY 11 - 7082 was used to treat the Ca- ki - 1 cells with KISS - 1 silence, the expression of MMP - 9 were detected by western blot. [ Results ] In Caki - 1 cells, KISS - 1 silence could up - regulate the expression of NF - kβ, and down - regulate the expression of MMP - 9 remarkably. After BAY 11 - 7082 was used to treat the Caki - 1 cells with KISS - 1 silence, the expression of MMP - 9 up - regulated remarkably. [ Conclusion] KISS - 1 gene can regulate the expression of MMP - 9 by NF -kβ in renal carcinoma cells.
作者 张辉 崔军 尚超 郭艳 宋永胜
机构地区 中国医科大学附属盛京医院泌尿外科 中国医科大学基础医学院神经生物学教研室 中国医科大学附属口腔医学院中心实验室
出处 《中国医学工程》 2012年第3期1-2,5,共3页 China Medical Engineering
基金 国家自然科学基金资助项目(30901480)
关键词 肾癌 NF-ΚB KISS-1 MMP-9 renal cancer, NF-kβ, KISS-1, MMP-9
分类号 R737.11 [医药卫生—肿瘤]
  • 相关文献

参考文献8

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二级参考文献12

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共引文献10

同被引文献26

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二级引证文献16

中国医学工程
2012年 第3期

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