适于子叶节和胚尖再生体系的大豆基因型筛选

Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems

以吉育91等6个基因型大豆的子叶节和胚尖为外植体,研究了大豆不同基因型在子叶节、胚尖2个再生体系中的不定芽诱导率、不定芽伸长率和不定芽数。结果表明:基因型对大豆不同再生体系的再生有显著影响,合丰35和合丰25在子叶节体系中的不定芽诱导率和伸长率均高于胚尖体系,而黑农37的不定芽诱导率和伸长率在胚尖体系高于子叶节体系。不同基因型适合的大豆再生体系不同,合丰35、合丰25和绥农14适合选用子叶节再生体系,黑农37更适合选用胚尖再生体系,而吉育91和北京小粒豆则既可以选择子叶节再生体系又可选择胚尖再生体系。

Cotyledon nodes and embryonic tips of soybean were used as explants to investigate the induction rate of adventitious shoots,elongation rate of adventitious shoots and the number of adventitious shoots.The results showed that genotype could affect regeneration frequency obviously in the selected regeneration system.For Hefeng35 and Hefeng25,both induction rates and elongation rates in cotyledon node regeneration system were higher than those in embryonic tip regeneration system,while the induction rates and elongation rates of Heinong37 were opposite.Different regeneration system should be applied when different soybean genotypes were used.Hefeng35,Hefeng25 and Suinong14 were suitable for cotyledon node regeneration system,Heinong37 was more suitable for the embryonic tip regeneration system.Furthermore,Jiyu91 and Beijingxiaolidou were suitable for both regeneration systems.