16SrRNA基因扩增在新生儿败血症诊断中的研究

Study on 16SrRNA amplification in the diagnosis of neonatal sepsis

目的:探讨建立新生儿败血症的分子生物学诊断方法。方法:分析16S rRNA核苷酸序列,设计引物及探针,选取临床常见菌株进行PCR扩增及检测;对疑为败血症的317例新生患儿分别行PCR和血培养检测。结果:PCR检测阳性率明显高于血培养(P<0.05)。以血培养为参照,PCR检测的敏感性高,特异性好。结论:建立了新生儿败血症的分子生物学诊断方法,满足临床快速、准确的要求,具有较大的推广及应用价值。

Objective：To develop a method in diagnosis of neonatal sepsis by molecular biology techniques.Methods：The primers and probes were designed and synthesized based on the sequences of 16S rRNA gene.The clinical isolated strains were detected by Polymerase chain reaction（PCR）.Blood specimens from 317 cases of suspected septicemia were cultured and 16S rRNA gene was detected by PCR separately.Results：The positive rate of PCR was significantly higher than that of blood culture（P0.05）.When blood culture was taken as control,the sensitivity and specificity of PCR were high.Conclusion：Molecular biology technique method for diagnosis of neonatal sepsis was developed with rapidity and accuracy.It may be valuable and practical in diagnosis of neonatal septicemia.