实时荧光定量PCR技术在副溶血性弧菌快速检测中的应用

Application of real-time quantitative PCR in the rapid detection of Vibrio parahaemolyticus in food and food poisoning samples

目的:建立副溶血性弧菌荧光定量PCR检测方法,应用于食品、食物中毒样本的快速检测。方法:食品样本先选择性增菌,取上层液提取DNA,粪样直接提取DNA,用实时荧光定量PCR方法进行检测,并与常规培养法进行比较。结果:用实时荧光定量PCR检测食品54份,食物中毒粪样12份,检测阳性食品21份、粪样9份,检测结果与培养法比较,阳性检出率分别为食品38.9%、31.5%;粪样均为75.0%。结论:副溶血性弧菌的实时荧光定量PCR检测方法具有快速、灵敏的优点。

Objective：The Vibrio parahaemolyticus quantitative PCR method was established for rapid detection of food and food poisoning samples.Methods：Selective enrichment of food samples was done at first,then DNA was extracted from upper liquid and stool samples,finally real-time quantitative PCR was used for detection and compared to the routine culture method.Results：54 food samples and 12 stool samples of food poisoning were detected by real-time PCR.The results showed 12 food samples and 9 stool samples were positive.Compared to the culture method,the positive rates of food samples were 38.9% for PCR and 31.5% for culture method,while that of stool samples were both 75.0%.Conclusion：Real-time quantitative PCR was an ideal method for rapid Vibrio parahaemolyticus detection with high accuracy and sensitivity.