前S1抗原和HBV-DNA检测结果的相关性分析

Analysis on detection of PreS1 and HBV-DNA

目的:探讨前S1抗原(PreS1)与HBV-DNA的相关性。方法:用酶联免疫法对450例慢性乙肝患者血清和95例健康人血清进行PreS1检测。用荧光定量PCR法检测HBV-DNA。结果:450例慢性乙肝患者血清中PreS1和HBV-DNA的阳性率分别为63.3%和74.4%。285例PreS1阳性标本中HBV-DNA的阳性数为270例,335例HBV-DNA阳性标本中PreS1阳性数为260例。结论:PreS1与HBV-DNA相关性较好,是乙型肝炎早期诊断和疗效评估的可靠指标。

Objective：To detect and analyze the correlation of PreS1 and HBV-DNA.Methods：PreS1 antigen in sera of 450 patients with chronic hepatitis B and 95 healthy persons was tested by ELISA,and HBV-DNA was detected by fluorescent quantitative PCR.Results：Among 450 patients with chronic hepatitis B,the positive rates of PreS1 and HBV-DNA were 63.3% and74.4%.Among 285 PreS1-positive samples,the positive cases of HBV-DNA were 270,while 260 PreS1 positive cases were found among 335 HBV-DNA positive samples.There weren′t significance difference between PreS1 and HBV-DNA（P0.05）.Conclusion：PreS1 was related to the result of serum HBV-DNA.PreS1 can reflect the infection and replication condition of HBV.PreS1 can be used as a reliable marker for early diagnosis and the efficiency of therapy in hepatitis B,which had important significance in clinic.