不同氢氧化钙制剂对牙髓卟啉单胞菌的杀菌效果

Antimicrobial effect of various calcium hydroxide on Porphyromonas endodontalis in vitro

目的:比较Endocal、Calxyl、国产氢氧化钙糊剂、Vitapex 4种氢氧化钙制剂对牙髓根尖周主要致病菌牙髓卟啉单胞菌(Porphyromonas endodontalis,P.e)活性的影响。方法:(1)通过动态比浊法比较在直接接触条件下不同氢氧化钙制剂不同作用时间对牙髓卟啉单胞菌的抑制作用。(2)选取新鲜拔除的人单根管牙85颗,经根管预备、高压消毒灭菌后,置入P.e菌悬液,建立牙髓卟啉单胞菌感染模型。将模型采用抽签法随机分为5组,即Endocal组、Calxyl组、国产氢氧化钙糊剂组、Vitapex组、无菌去离子水对照组。分别于封药前和封药7d后,用无菌纸捻在根管中取样,细菌培养计数菌落,并完成细菌24h回复实验。采用SPSS11.0软件包对数据进行统计学处理。结果:Endocal、Calxyl、国产氢氧化钙糊剂、Vitapex均能有效减少牙髓卟啉单胞菌的数量(P<0.05),细菌清除率均达95%以上。动态比浊法检测结果显示,Endocal、国产氢氧化钙糊剂抑菌作用优于其他组,有显著性差异(P<0.05);细菌培养法结果显示,Endocal组细菌清除率显著大于Vitapex组(P<0.05)。结论:Endocal、Calxyl、国产氢氧化钙糊剂、Vitapex 4种药物对牙髓卟啉单胞菌均有较好的抑制作用,其中,Endocal的抗菌作用优于Vitapex。

PURPOSE： To compare the antimicrobial activity of Endocal,calcium hydroxide paste,Calxyl,Vitapex on Porphyromonas endodontalis（P.e）.METHODS：（1） The antimicrobial activity of different calcium hydroxide on P.e was examined at different exposure times by dynamic nephelometry.（2） 85 freshly extracted single-rooted human teeth were selected and cut at the amelocemental junction.All roots were randomly divided into five groups.The bacteria were incubated in each canal and were sampled and counted before and after enveloping five kinds of intercanal medicine seeded.Student’s t test,One-way ANOVA were used with SPSS11.0 software package for statistical analysis.RESULTS： The bacteria from each group were reduced significantly after intracanal medication（P〈0.05）.The antibacterial efficacy of Endocal and calcium hydroxide paste were superior to others under dynamic nephelometry test（P〈0.05）.Endocal,calcium hydroxide paste,Calxyl,Vitapex had strong inhibitory effect on P.e from infected root canals,and the rate of bacteria clearance was 95%.The antimicrobial activity of Endocal was significantly greater than others（P〈0.05）.CONCLUSIONS： Endocal,calcium hydroxide paste,Calxyl and Vitapex were effective for intercanal disinfection.The antibacterial activity of Endocal is greater than Vitapex.