用双色荧光原位杂交法检测苯系物接触工人精子染色体数目畸变

Detection of numerical chromosome aberrations in sperm of workers exposed to benzene series by two color fluorescence in situ hybridization

目的 探讨苯系物对男工精子性染色体数目畸变的影响。方法 用X、Yα 卫星染色体特异DNA探针与苯系物接触男工间期精子核进行双色荧光原位杂交 ,同时测定X和Y精子染色体非整倍体率。结果 车间空气中苯浓度几何均数 83 95mg m3 高于国家最高容许浓度 1倍。对 13位苯系物作业工人共计数 74 5 17条精子 ,X、Y和XY精子双体率分别为 ( 0 15± 0 0 5 ) %、( 0 13± 0 0 5 ) %和( 0 2 0± 0 11) % ;13位正常对照工人共计数 89662条精子中X、Y及XY精子双体率分别为 ( 0 10±0 0 4 ) %、( 0 10± 0 0 4 ) %和 ( 0 13± 0 0 6) %。接触组精子X双体率及总双体精子率高于对照组。结论 高浓度苯接触可以诱导接触工人精子性染色体非整倍体率增加。

Objective Tostudythenumericalaberrationsofsexchromosomesinspermofworkers exposedtobenzeneseries.Methods Theinterphasespermfromworkersofexposedandcontrolgroupswere hybridizedusingtwocolorfluorescenceinsituhybridizationwithXandYα satellitechromosomespecificDNA probes ,andtheaneuploidyfrequenciesofXandYchromosomeinXandYspermsweredetectedsimultaneously. Results Geometricmeanconcentrationofbenzeneinworkplaceairwas 83 95mg m3 ,onefoldhigherthan nationalmaximumallowableconcentration .Atotalof 74 5 17spermnucleiin 13benzene exposedworkersand 89 662spermnucleiin 13controlworkerswerecounted .FrequenciesofdisomicspermforX ,YandXYwere ( 0 15± 0 0 5 ) % ,( 0 13± 0 0 5 ) %and ( 0 2 0± 0 11) %inworkersexposedtobenzeneseries,and ( 0 10± 0 0 4 ) % ,( 0 10± 0 0 4 ) %and ( 0 13± 0 0 6) %incontrolworkers .Therewasstatisticaldifferenceindisomic frequencyofXspermandoveralldisomicfrequencybetweenthebenzeneseries exposedworkers ( 0 15 %and 0 4 8% ,respectively)andthecontrolworkers( 0 10 %and 0 3 3 % ,respectively) .Conclusion Exposedto benzeneathigherconcentrationmayinduceincreaseinaneuploidyfrequencyofspermsexchromosomeinexposed workers.