鹅肌肉IGF-Ⅰ基因mRNA的表达

Study on the Changes of IGF-ⅠmRNA Expression Levels in the Developmental Muscles of the Geese

以吉林白鹅肉用品系、蛋用品系和霍尔多巴吉白鹅为供试材料,采用实时定量PCR法对胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因在鹅1、30、60、90日龄时胸肌、腿肌中mRNA的表达水平进行测定分析。结果表明:鹅胸肌、腿肌IGF-ⅠmRNA的表达量随着日龄的增加呈先升后降的趋势,胸肌中IGF-ⅠmRNA的表达峰值在吉林白鹅肉用品系60日龄、蛋用品系30日龄、霍尔多巴吉白鹅60日龄出现,其中霍尔多巴吉白鹅30日龄与1日龄差异不显著(P>0.05);腿肌IGF-ⅠmRNA的表达量均在30日龄达到最大,随后下降。在胸肌组织中,吉林白鹅肉用品系IGF-ⅠmRNA的表达量在1、60和90日龄时均高于蛋用品系,霍尔多巴吉白鹅IGF-ⅠmRNA表达量在1日龄和90日龄时高于吉林白鹅蛋用品系(P<0.05),30日龄品种间差异不显著(P>0.05)。在腿肌组织中,吉林白鹅肉用品系IGF-ⅠmRNA在30和60日龄的表达量均高于霍尔多巴吉白鹅,在60和90日龄高于吉林白鹅蛋用品系(P<0.05);霍尔多巴吉白鹅在30日龄时IGF-ⅠmRNA表达量低于蛋用品系(P<0.05),90日龄时高于吉林白鹅两个品系(P<0.05),1日龄时表达水平无差异(P>0.05)。

In this study,we detected the expression of IGF-I mRNA at 1（D1）,30（D30）,60（D60）,and 90（D90） days of age in the breast muscle and leg muscle of geese from meat-type Jilin goose,egg-type Jilin goose and hortobagyi goose.We found that the expression of IGF-Ⅰ mRNA in skeletal muscle tissues differed based on developmental age.The expression of IGF-Ⅰ mRNA exhibited a trend that ascended first and then descended after birth.IGF-Ⅰ mRNA was expressed in all breast muscle tissues during each stage with higher levels in meat-type goose and hortobagyi goose than that in egg-type Jilin goose（P0.05）.The expression pattern of IGF-Ⅰ mRNA in breast muscle of meat-type goose was similar to that in and hortobagyi goose,and in leg muscle,IGF-Ⅰ mRNA expression patterns were all down-regulated from D30.Similarity in IGF-Ⅰ mRNA expression pattern was found in breast among meat-type jilin goose,egg-type jilin goose and hortobagyi goose,which indicates that IGF-Ⅰ may be related to skeletal muscle differentiation and growth and suggests IGF-Ⅰ mRNA maybe tissue-specific expression and individual difference.