用荧光分析法探讨粘结性偶联剂与牙面的结合机制

STUDIES ON BONDING MECHANISM BETWEEN ADHESIVE COUPLING AGENT AND HUMAN TOOTH SURFACE BY FLUORESCENT ANALYSIS

用固体荧光分别测得牙本质、牙釉质的荧光光谱。以具荧光性和具理论上可与钙离子化合的自制粘结性偶联剂(用作荧光探针)处理牙面后,测得固体荧光峰位发生明显红移。结果证明,偶联剂与牙体组织之间发生了化学结合而产生了新态。又根据溶液中偶联剂与钙离子作用后的荧光峰位红移的现象及测得络合比为2:1,推论偶联剂与牙面钙发生了螯合作用。

<ABSTRACT> In order to study the bonding mechanism between the bonding agent and human tooth surface, we adopt the fluorescence probe method and synthesize adhesive coupling agent (NTG-CGE (N ),CGE(N) -1)used as a probe of having fluorescence and excited complex (in solution ) characteri- stics. The results indicate that: 1)human dentin and enamel have their own fluorescence spectra (Fig 1,2 ), the fluorescent intensity of fresh tooth being higher than that of tooth in store, but their excitation and emission tvave lengths being identical and the fluorescent characteristics of human tooth being related to hydroxyapatite Fand 2)after the tooth surface is pri- med with the adhesive coupling agent in acetone solution, the solid fluo- resence spectra change obviously (red shift,Tables 1,2; Fig 3,4 ),and after the coupling agent being added to Ca2+ in the solution, the fluorescence spectra of the solution also change obviously with disappearance of spectrum of excited complex (Fig 5,6 ), the ratio of central Ca2+ ion to the ligand(NTG-CGE (N)or CGE (N ) -1) being 1:2 (Fig 7,8 ). By these ph- enomena, it is proved cxplicitly that there is chemical reaction between tooth surface and the coupling agent, and we can deduce that the coupling agent chelates with Ca2+ of tooth surface.