阿托伐他汀对脓毒症大鼠血浆IL-1β的影响

Effects of atorvastatin on level of IL-1β in plasma of rats with sepsis

[目的]探讨阿托伐他汀对脓毒症大鼠血浆IL-1β浓度及宿主炎症反应的影响。[方法]选择健康雄性S-D大鼠100只,采用盲肠结扎穿孔法(cecal ligation and puncture,CLP)制造大鼠脓毒症模型,随机分为对照组、脓毒症组、阿托伐他汀低剂量组(20 mg/kg.d)与高剂量组(40 mg/kg.d),依据改良实验动物脓毒症严重程度评定标准评分,于术后0 h,3 h,6 h,12 h和24 h各取5只大鼠采血,用ELISA方法检测血浆IL-1β浓度,比较各组间大鼠脓毒症严重程度评分及血浆IL-1β浓度。[结果]在0 h,脓毒症严重程度评分各组间比较差异不显著,对照组各时间点评分无明显变化,但在3 h、6 h、12 h和24 h各时间点该评分按脓毒症组、阿托伐他汀低剂量组及高剂量组依次降低,各组间差异有显著性意义(P<0.01)。在0 h,血浆IL-1β浓度在各组间差异无显著性意义(P>0.05),但在3 h、6 h、12 h和24 h各时间点按脓毒症组、阿托伐他汀低剂量组,阿托伐他汀高剂量组依次降低,组间差异明显(P<0.01)。[结论]阿托伐他汀抑制脓毒症IL-1β释放,可能对治疗脓毒症有所帮助。

[Objective] To investigate the influence of atorvastatin on interleukin-1β（IL-1β） in plasma and inflammatory response of rats with sepsis.[Methods] Sepsis models were made with male SD rats.The sepsis models of rats were manufactured by cecal ligation and puncture（CLP）.A total of 100 healthy rats were divided randomly into 4 groups（n=25） including sham-operation,CLP,low-dose atorvastatin（20 mg/kg·d） and high-dose（40 mg/kg·d） groups.The sepsis severities of the model animals were scored according to modified sepsis severity assessment standards of experimental animals.Blood samples from five rats in each group were collected to detect IL-1β concentration in plasma with ELISA at postoperative 0,3,6,12 and 24 hours.We compared the sepsis severity score and IL-1β concentration in plasma of the rats among these groups.[Results] At 0 h,the sepsis severity scores did not have significant difference among the four groups（P0.05）.The variation of scores was not obvious in sham-operation group at the four time point,but at 3 h,6 h,12 h and 24 h,the scores in CLP,low-dose atorvastatin and high-dose atorvastatin groups decreased gradually.There was significant difference among the three groups（P0.01）.At 0 h,IL-1β levels of plasma did not have significant difference among the four groups（P0.05）.However at 3 h,6 h,12 h and 24 h,the IL-1β levels in plasma in CLP,low-dose atorvastatin and high-dose atorvastatin groups decreased gradually.There was significant difference among the three groups（P0.01）.[Conclusions] Atorvastatin may be helpful for treatment of sepsis due to its ability to inhibits the release of IL-1β in sepsis rats.