摘要
目的通过白术多糖的含量测定研究,比较苯酚-硫酸和蒽酮-硫酸两种常用测定多糖的方法。方法通过精密度试验,稳定性试验,回收率试验等方面考察苯酚-硫酸法和蒽酮-硫酸法多糖测定方法。结果苯酚-硫酸法测定白术多糖含量优于蒽酮-硫酸法。结论苯酚-硫酸法测定白术多糖含量,不需要精密仪器,操作简便,稳定可靠,适合白术及其制品中多糖的常规检测,可为植物多糖含量测定方法选择提供参考。
Objective To select a more accurate and convenient method of determining Crude Atractylodes macrocephala Koidz.Polysaccharide（CAMP） content.Methods The content was determined by two method of phenol sulfuric acid colorimetric method and anthranone sulfuric acid method.Results The comparison showed phenol-sulfuric acid method was more stable and accurate than phenol sulfuric acid colorimetric method.The recovery was higher.Conclusion So the better method to determine the content was phenol sulfuric colorimetric acid.
出处
《现代中药研究与实践》
CAS
2012年第2期73-75,共3页
Research and Practice on Chinese Medicines
基金
湖南省自然科学基金资助课题(编号08jj3024)
长沙市科技管理局(K069062-12)
湖南省医药卫生科研资助课题(B2007119)
关键词
白术粗多糖
蒽酮硫酸法
苯酚硫酸法
Atractylodes macrocephala Koidz.
polysaccharide
anthranone sulfuric acid method
phenol sulfuric acid colorimetric method
