紫癜灵颗粒提取工艺优选

Optimization of Extraction Process for Zidianling Granule

目的:优选中药紫癜灵颗粒的提取工艺。方法:以黄芩苷和苦参碱作为评价指标,采用L9(34)正交试验对影响提取工艺的主要因素进行优选,HPLC测定黄芩苷和苦参碱含量。结果:紫癜灵颗粒优选提取工艺为黄芩等5味药用6倍量70%乙醇回流提取2次,每次1 h;药渣及水牛角等余药加10倍量水煎煮3次,每次1.5 h。结论:正交试验优选出的提取条件经济、合理,适合批量生产。

Objective：To optimize extraction technology of Zidianling granule.Method：With baicalin and matrine as indicators,main factors of extraction process were optimized by L9（34）orthogonal test,the content of baicalin and matrine were determined by HPLC.Result：Optimum extraction process was determined as follows： Scutellaria baicalensis and other five herbs was extracted 2 times with 6 times the amount of 70% ethanol,1 hour each time;Residues of above mentioned drugs and the others herbs（Bubalus bubalis and the others herbs） were extracted 3 times with 10 times the amount of wate,1.5 h each time.Conclusion：This optimized technology for Zidianling granules was economic,stable and suitable for mass production.