多指标正交试验优选麻黄附子细辛汤提取工艺

Optimization of Extraction Technology for Mahuang Fuzi Xixin Decoction by Multi-index Orthogonal Test

目的:优选麻黄附子细辛汤提取工艺。方法:采用HPLC测定指标成分含量,以麻黄碱、伪麻黄碱、苯甲酰乌头原碱、苯甲酰新乌头碱、苯甲酰次乌头碱和干浸膏得率为检测指标,采用L9(3)4正交试验考察提取时间、提取次数和溶剂用量对提取工艺的影响。结果:麻黄附子细辛汤最佳提取工艺为16倍量pH 2.0盐酸提取2次,每次2.5 h。结论:多指标综合评定优化提取工艺更符合中医用药的整体观,优化的提取工艺方法合理可行,稳定可靠,具可操作性和重复性。

Objective：To optimize extraction technology of Mahuang Fuzi Xixin decoction.Method：With ephedrine,pseudoephedrine,benzoyl aconine,benzoyl mesaconitine,benzoyl hypaconitine and yield of extract as indexes,the content of these were determined by HPLC,effect of extraction time,extraction times and the amount of solvents on extraction technology was investigated by L9（3）4 orthogonal test.Result：Optimum extraction conditions were：extracted 2 times with 16 times the amount of hydrochloric acid（pH 2.0） with 2.5 hours each time.Conclusion：It was more suitable for integral view of Chinese medicine by multi-index comprehensive evaluation method,this optimized extraction technology was reasonable,feasible,stable,reliable,operable and good reproducibility.