西北地区商品甘草HPLC指纹图谱

HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China

目的:研究西北地区商品甘草的高效液相指纹图谱,为建立商品甘草的行业质量标准提供依据。方法:采用高效液相色谱法,选用ZORBAX Edipse XDB-C18柱(4.60 mm×250 mm,5μm),柱温25℃,以乙腈-0.05%磷酸溶液为流动相,检测波长237 nm,进行梯度洗脱,生成图谱。使用"中药色谱指纹图谱相似度评价系统2004A"和SPSS软件建立HPLC对照指纹图谱并进行聚类分析。结果:建立了西北地区商品甘草的高效液相指纹图谱,方法学验证符合要求。16批商品甘草药材的指纹图谱有10个特征共有峰,与生成对照图谱比较相似度均>0.904,通过聚类分析将16批药材聚为4类。结论:该方法稳定、可靠,本研究首次采用HPLC指纹图谱比较西北地区不同等级的商品甘草,为西北地区商品甘草的质量考察、等级划分提供有益参考。

Objective： Research the HPLC fingerprint of the pieces of commercial Glycyrrhizae Radix from the north-west China,and establish the quality standards of commercial Glycyrrhizae Radix to provide a basis for the industry.Method： High performance liquid chromatography was used for the soparation on.Choice ZORBAX Edipse XDB-C18 column（4.60 mm×250 mm,5 μm）,column temperature was maintained at 25 ℃,with acetonitrile-0.05% phosphoric acid was selected as mobile phase by gradient elution,the detection wavelength was set at 237 nm,the gradient elution were adopted.Using the ＇chromatographic fingerprint evaluation system 2004A＇ and SPSS software was used to establish HPLC fingerprint comparison and cluster analysis.Result： Established the HPLC fingerprint of the pieces of commercial Glycyrrhizae Radix from the north-west area China was established,and the results of methodological study met the technical requirements for fingerprinting.Ten common peaks were found in the fingerprint of Sixteen batches of commercial Glycyrrhizae Radix,the similarity was greater than 0.904,and four groups were divided by cluster analysis,together into four groups.Conclusion： This is the first use of HPLC fingerprint to compare different levels of commercial Glycyrrhizae Radix from the north-west China.The HPLC method is stable and reliable to provide a scientific basis of quality inspection and classification for the commercial Glycyrrhizae Radix.