乌鸡白凤丸对小鼠前列腺增生模型的影响

Effect of Wuji Baifeng Wan on Mice Model of Benign Prostate Hyperplasia

目的:探讨乌鸡白凤丸对小鼠前列腺增生模型的影响。方法:采用昆明种小鼠去势,连续3周sc丙酸睾酮5mg·kg-1·d-1,造前列腺增生模型。将模型小鼠分为5组,分别ig 9,4.5,2.25 g·kg-1的乌鸡白凤丸混悬液,450 mg·kg-1癃闭舒胶囊混悬液和同体积的生理盐水,另设一组空白组给同体积生理盐水;每天给药1次,连续给药30 d。末次给药2 h后处死小鼠,观察乌鸡白凤丸对前列腺增生模型小鼠血清睾酮、雌二醇水平,前列腺指数及前列腺、胸腺组织形态的影响。结果:与前列腺增生模型组比,乌鸡白凤丸9,4.5,2.25 g·kg-1剂量可显著降低前列腺增生模型小鼠血清睾酮水平、显著升高血清雌二醇水平,血清睾酮水平及雌二醇水平分别为(2 713.2±1 282.8),(3 767.2±1 044.9),(4 350.2±1 632.9)nmol·L-1及(131.9±36.0),(78.4±34.9),(80.2±26.2)nmol·L-1。乌鸡白凤丸9,4.5,2.25 g·kg-1剂量可显著降低模型小鼠前列腺指数,使前列腺增生显著减轻,前列腺腺体密度分别为(5.1±0.4),(4.2±0.3),(6.8±0.4);乌鸡白凤丸9,4.5,2.25 g·kg-1剂量可使胸腺显著增厚,淋巴细胞数显著增多。结论:乌鸡白凤丸对丙酸睾酮所致去势小鼠前列腺增生有好的治疗作用。

Objective：To explore the effect of Wuji Baifeng Wan on benign prostate hyperplasia（BPH） in mice.Method：Experimental prostatic hyperplasia mouse model was produced in the gonadectomy mouse by hypodermic injecting testosterone propionate 5 mg·kg-1·d-1 for 30 d.The model mice were divided into 5 groups,respectively were fed with 9,4.5,2.25 g·kg-1 Wuji Baifeng Wan suspension,450 mg·kg-1 longbishu capsule suspension and the same volume of normal saline solution.The blank control group was fed with the same volume of normal saline solution.Every group was given daily for 30 days.At two hours after the last administration,mice were sacrificed.The serum testosterone and estradiol levels,the prostate index and histomorphology changes of prostate gland,thymus gland that were observed.Result：Compared with model group,the Wuji Baifeng Wan at the dosage of 9,4.5,2.25 g·kg-1could reduce the testosterone and increase the estradiol levels observably.Levels of testosterone and estradiol in different groups were（2 713.2±1 282.8）,（3 767.2±1 044.9）,（4 350.2±1 632.9） nmol·L-1 and（131.9±36.0）,（78.4±34.9）,（80.2±26.2） nmol·L-1.Wuji Baifeng Wan at the dosage of 9,4.5,2.25 g·kg-1 could reduce the prostate indexes and alleviate the BPH observably.The volume density of prostate glands in different groups were（5.1±0.4）,（4.2±0.3）,（6.8±0.4）.Wuji Baifeng Wan at the dosage of 9,4.5,2.25 g·kg-1 could incrassate thymus gland and increase the number of lymphocyte observably.Conclusion：Wuji Baifeng Wan have good therapeutic actions on gonadectomy BPH model caused by testosterone propionate in mice