板蓝根不同提取部位的体外抗氧化活性

Antioxidation of Different Extracts from Isatidis indigotica in vitro

目的:考察板蓝根不同提取部位的体外抗氧化活性。方法:用大孔树脂和阴、阳离子交换树脂制备板蓝根3个有效部位:总木脂素、总有机酸、总生物碱部位。结合酶标仪,通过清除1,1-二苯基苦基苯肼(DPPH)自由基和Fe3+还原能力(FRAP)测定板蓝根提取物的体外抗氧化作用。结果:板蓝根颗粒组及各个提取物组均有不同程度的体外抗氧化效果。其中以总木脂素抗氧化效果最佳(DPPH IC50160.6 mg·L-1;FRAP 0.75 mmol·g-1);其次为板蓝根颗粒组(DPPH IC50595.97 mg·L-1;FRAP 0.12 mmol·g-1);总有机酸,总生物碱部位效果较弱。结论:测定了板蓝根中不同有效部位的抗氧化活性,初步确定其抗氧化相关活性成分主要存在于木脂素部位。

Objective： Antioxidation of different extracts from Isatidis indigotica were studied in vitro to supply the separating base of antioxidative components.Method： The extracts were prepared by macroporous resin and anion/cation exchange resin.Their antioxidant activities were assessed by two complementary test systems,namely 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging activity and ferric reducing antioxidant power（FRAP） assay.Result： The total lignin displayed strong activity in all test systems.（DPPH IC50 160.6 mg·L-1;FRAP 0.78 mmol·g-1） Banlangen Keli（DPPH IC50 595.97 mg·L-1;FRAP 0.12 mmol·g-1） showed relatively lower activity.Total organic acids and total alkaloid have a little activity.Conclusion： Antioxidative components could be justified existing in the total lignin initially.