摘要
建立了一种简单、快速、可靠的测定乳制品中叶黄素的新方法。乳制品中的叶黄素经正己烷提取,氮气吹至近干,残余物用正己烷-乙酸乙酯(70+30)溶解后,高效液相色谱法测定,标准曲线法定量。该方法具有良好的准确度和精密度,在不同乳制品中添加回收率范围为91.0%～102.6%,相对标准偏差(RSD)为0.64～2.92。该方法的检出限和最低定量限分别为20μg/kg和50μg/kg。
A simple fast and reliable method has been developed for the determination of lutein in dairy products. The samples were extracted by n-hexane, blown nearly dry with nitrogen. The residue was dissolved by n-hexane-ethyl acetate （70＋30）, and the final solution was determined by HPLC. The method was quantified by calibration curve method. The method has good accuracy and precision. The recovery of lutein in dairy products was 91.0%-102.6%. The relative standard deviations （R.SD） was 0.64-2.92. The detection limits oflutein was 20 μg/ kg, and the quantification limits was 50μg/kg.
出处
《中国乳品工业》
CAS
北大核心
2012年第4期48-50,共3页
China Dairy Industry
关键词
乳制品
叶黄素
高效液相色谱法
dairy products
lutein
high performance liquid chromatography
