猪肺疫多杀性巴氏杆菌的分离鉴定

Isolation and identification of Pasteurella multocida isolated from swine pneumonic pasteurellosis

为了研究四川乐山某猪场爆发的保育猪严重呼吸道疾病的主要病原,试验采用病理剖检、病毒检测、细菌分离鉴定、生化鉴定、动物回归试验以及多杀性巴氏杆菌种特异性KMT基因的PCR检测对病原进行鉴定。结果表明:发病猪有严重肺炎症状,支气管内部有少量泡沫,腹股沟淋巴结出血、水肿,其他实质器官未见明显病理变化;病毒检测结果显示为蓝耳病病毒阳性,猪瘟病毒、伪狂犬病毒、圆环病毒2型阴性;从病猪肺脏中分离获得大肠杆菌和1株蓝耳病病毒继发的高致病性多杀性巴氏杆菌;该分离菌株对多种药物均高度敏感。

To research the major pathogens which caused serious respiratory diseases in nursery pigs outbroken in a pig farm in Leshan in Si- chuan province, the pathogens were identified using pathological anatomy, virus detection, isolation and identification of bacteria, biochemical identification, regression experiment for aniaml and PCR identification of species - specific KMT gene of PasteurcUa multocida. The results showed that the symptoms of severe pneumonia appeared in diseased pigs, with a small amount of foam in the internal bronchial tube , hemor- rhage and edema in inguinal lymph nodes, and other parenchymatous organs had no obvious pathological changes. The result of the virus detec- tion was positive with blue ear disease, swine fever, pseudorabies and PCV - 2 negative. E. coli and a strian of Pasteurella muhocida with high pathogenicity caused by secondary blue - ear disease were isolated from the lungs in pigs. Drug susceptibility testing showed that the isolate was highly sensitive to a variety of drugs.