原发性肝细胞癌组织中Connexin43和CD147的表达及意义

Expression of Cx43 and CD147 and their significance in primary hepatocellular carcinoma

目的探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)中Connexin43(Cx43)和CD147蛋白的表达及意义。方法采用免疫组化PV6000法检测74例HCC和20例正常肝组织中Cx43和CD147的表达。结果 Cx43和CD147在HCC组织中阳性率分别为27.03%、63.51%,在正常肝组织中阳性率分别为95.00%、30.00%,Cx43在正常肝组织中的阳性率明显高于HCC组织中的阳性率,差异有显著性(P<0.01);CD147在HCC组织中阳性率明显高于正常肝组织的阳性率,差异有显著性(P<0.01)。Cx43和CD147阳性表达与肝细胞癌的肿瘤大小、分化程度、TNM分期及肝内血管癌栓具有相关性(P<0.05),与患者的年龄、性别、有无伴肝硬化、门静脉癌栓及术前血清甲胎蛋白水平无相关性(P>0.05)。肝癌组织中Cx43表达下调,CD147表达上调,两者呈负相关(P<0.01)。结论联合检测HCC组织中Cx43和CD147的表达情况,可预测HCC的浸润和转移,对指导临床治疗和判断预后有重要意义。

Purpose To investigate the expression of Cx43 and CD147 in primary hepatocellular carcinoma（HCC） and their relationship with clinicopathological features.Methods PV6000 immunohistochemical system was used to detect the expression of Cx43 and CD147 in 74 HCC tissues and 20 normal liver samples.Results The positive expression rates of Cx43 and CD147 protein in HCC were 27.03% and 63.51%,respectively.However,their positive rates in normal liver tissues were 95.00% and 30.00%,respectively.There was a significant difference between HCC and normal liver tissues（P0.01）.The expressions of Cx43 and CD147 were associated with tumor size,differentiation grade,TNM stages and the introhepatic vascular cancer embolus（P0.05）.However,their expressions were not related with age,gender,with or without cirrhosis background,the portal vein cancer embolism and the serum level of alpha-fetal protein（AFP）（P0.05）.Down-regulation of Cx43 and up-regulation of CD147 were negatively correlated in HCC（P0.01）.Conclusions Detection of both factors Cx43 and CD147 in HCC is useful for predicting the local invasion and metastasis.They could be used as novel and useful markers to direct clinic therapy and estimate the prognosis.