摘要
目的探讨Mina53在非小细胞肺癌(non small cell lung carcinoma,NSCLC)中的作用及Mina53、增殖细胞核抗原(PC-NA)在NSCLC中联合表达的意义。方法采用免疫组化PV-9000两步法、Western blot法检测46例NSCLC和10例正常肺组织中Mina53、PCNA蛋白的表达情况。结果在正常肺组织中Mina53、PCNA表达量均很少,而在NSCLC中均高度表达。Mi-na53与PCNA表达均与组织分化、临床分期、淋巴结转移有明显的相关性,Mina53、PCNA蛋白之间具有明显的正相关性。结论 Mina53、PCNA相互影响、相互作用,在肺癌的发展过程中起促进作用。
Purpose To explore a possible role of Mina53 protein and the significance of the coexpression of Mina53 and PCNA proteins in non small cell lung carcinoma.Methods Immunohistochemical methods and Western blot were used to examine the expression of Mina53,PCNA in non small cell lung carcinoma tissues（n=46） and normal lung tissues（n=10）.Results There were little expression of Mina53,PCNA in normal lung tissues,while there were higher expression of both markers in non small cell lung carcinoma tissues.Mina53 and PCNA were significantly associated with histological differentiation,clinical stages,and lymph node metastasis.Mina53 and PCNA proteins were positively related.Conclusion The coexpression of Mina53 and PCNA proteins plays an important role in the pathogenesis of non small cell lung carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第4期390-393,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
湖北省教育厅科学研究重点项目(2009-55)
