植物甜菜碱醛脱氢酶的生物信息学分析

Bioinformatics Analysis of Betaine Aldehyde Dehydrogenase in Plants

运用生物信息学的方法,对已在GenBank数据库中注册的甜菜、菠菜、山菠菜、玉米、辽宁碱蓬等植物甜菜碱醛脱氢酶(BADH)的氨基酸序列进行分析。结果表明:植物BADH属于稳定蛋白质,含有较丰富的丙氨酸和谷氨酸;不同植物BADH的氨基酸序列具有较高的同源性,含有与酶功能相关的十肽保守序列及半胱氨酸残基;不同植物的BADH具有不同的亚细胞定位;分子进化研究表明,BADH可作为植物遗传分化和分子进化研究的重要依据;氨基酸序列中不存在信号肽;分子中不存在跨膜结构域,可能受蛋白激酶的磷酸化;α?螺旋是多肽链中的主要结构元件;蛋白质保守区域包含典型的醛脱氢酶功能结构域。该研究结果为开展BADH的酶学特性及植物抗逆的分子机理研究提供理论参考。

Bioinformatic methods were employed to analyze the amino acid sequences of Betaine Aldehyde Dehydrogenase（BADH） from Genbank database,such as Beta vulgaris,Spinacia oleracea,Atriplex hortensis,Suaeda liaotungensis,Zea mays,etc.The results showed that BADHs were stable proteins.Ala and Glu were abundant in those BADHs.Sequence alignment revealed that BADHs were highly homologous in different plants,and the residues involved with function were very conservative.BADHs had been found to be targeted to different subcellular compartments.BADHs could be used as genetic differentiation and an important basis for the study of molecular evolution.There was no signal peptide in segmental plants.BADHs had no transmembrane structure and could be phosphorylated by protein kinase.α？helix were the major structural element of polypeptide chain.Meanwhile,BADHs contained typical function domains of ALDH.This study provided a theoretical basis for both the enzymatic mechanism of BADHs and the plant resistance to environmental stress.