肠道病毒71型抵抗I型干扰素诱导的抗病毒作用

Human enterovirus 71 （EV71） resisted type I interferon induced antiviral effect

目的研究肠道病毒71型（EV71）抵抗I型干扰素（interferon，IFN）诱导的抗病毒作用。方法将1000U／ml的I型干扰素（α，β）加入HeLa细胞后，去除上清中的干扰素，感染带有GFP的重组单纯疱疹病毒（HSV-1）和EV71，观察GFP的表达和PCR检测HSV-1核酸，判断I型干扰素对HSV，1的作用。通过RT—PCR方法检测EV712A基因的表达从而判断EV71病毒的复制能力。结果I型干扰素（α，β）诱导HeLa细胞产生抗病毒蛋白而有效地抑制重组HSV-1GFP的表达和核酸扩增。而EV712A的RT—PCR结果证实EV71可在I型干扰素（α，β）作用后的HeLa细胞中有效增殖。结论I型干扰素（α，β）诱导产生抗病毒蛋白；EV71可在I型干扰素（α，β）诱导产生抗病毒蛋白的HeLa细胞中有效增殖。

Objective To investigate human enterovirus 71 （ EV71 ） resistance to type I interferon induced antiviral effect. Methods After type I interferons （α,β） were incubated with HeLa cells, recombinant type I herpes simple virus （HSV-1） with green fluorescent protein （GFP） was inoculated onto the HeLa cells. HSV-1 proliferation was observed by GFP expression and PCR. After EVT1 was inoculated onto HeLa cells incubated with the same quantity of interferon, proliferation of EV71 were detected by RT- PCR of 2A gene. Results Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type I interferon （α,β）. However, EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR. Conclusion HcLa cell irritated by type I interferon （α,β） produced antiviral substance that inhibits HSV-1 proliferation. EVT1 resisted the antiviral substance induced by type I interferon and could significantly replicate in the HeLa cells.