摘要
目的探讨microRNA-125b(miR-125b)基因在胃癌患者组织中的表达改变情况,及其对胃癌细胞系增殖和凋亡的影响。方法使用real-time PCR方法检测miR-125b在40例临床诊断为胃癌患者的癌组织与癌旁对照组织中的表达情况。随后使用miR-125b mimic转染胃癌细胞系HGC-27和MGC-803,确认过表达成功后,分别使用CCK-8试剂盒和流式细胞仪检测过表达miR-125b对细胞增殖和凋亡的影响。结果证实在胃癌患者组织中miR-125b的表达水平显著高于癌旁对照组(P<0.01)。在胃癌细胞系HGC-27和MGC-803中过表达miR-125b后,细胞增殖明显增加:转染72 h,HGC-27(scramble组:1.632±0.09,mimic组:2.473±0.08),MGC-803(scramble组:1.603±0.05,mimic组:2.554±0.07)),同时细胞凋亡也受到抑制。结论 miR-125b可能作为癌基因在胃癌中发挥作用,并对细胞增殖和凋亡具有显著影响。
Objective To evaluate the expression level of microRNA-125b(miR-125b) in gastric cancers and study its roles in cell proliferation and apoptosis.Methods The expression of miR-125b was detected by real-time PCR in 40 gastric cancer tissues and paired cancer-adjacent tissues.The synthesized miR-125b mimic was transfected into gastric cancer cell lines HGC-27 and MGC-803.The cell proliferation and apoptosis were measured by CCK-8 and flow cytometry respectively.Results MiR-125b was significantly up-regulated in gastric cancer tissues compared with cancer-adjacent controls(P0.01).Ectopic expression of miR-125b in HGC-27 and MGC-803 cells increased cell proliferation,while decreased cell apoptosis.Cells transfected for 72 h,HGC-27(scramble:1.632±0.09,mimic:2.473±0.08),MGC-803(scramble:1.603±0.05,mimic:2.554±0.07)).Conclusions MiR-125b may act as an oncogene in gastric cancer through regulation of cell proliferation and apoptosis.
出处
《基础医学与临床》
CSCD
北大核心
2012年第5期500-504,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(91129716)