ULBPs及MIC分子在亚健康状态诊断及评价中的作用

Roles of ULBPs and MIC molecules in the diagnosis and evaluation of sub-health status

目的建立亚健康状态人群ULBPs和MICA/B分子表达数据库,分析它们作为亚健康状态的具体参考指标的可能。方法用体检和问卷调查的方法对志愿者进行筛查,用酶联免疫技术检测血清中ULBPs和MICA/B含量。结果亚健康组的MICA、MICB、ULBP1和ULBP2的含量均高于健康对照组,而ULBP3、ULBP4和ULBP5低于健康对照组。亚健康组和健康组之间只有MICB有显著性差别(P<0.01)。另外,男性MICA和ULBP3的平均值显著高于女性。而ULBP2和ULBP4会随着年龄的增长呈现上升趋势。结论亚健康组的血清MICB含量明显升高,它可以联合其他免疫指标一起作为诊断亚健康的一种辅助手段。

Objective To establish a database of ULBPs and MICA/B molecule express level in peripheral blood of sub-health state population,and provide a reference for evaluation the health of overall population and prevention of diseases.Methods The health statuses of volunteers were evaluated according to physical examination and questionnaire survey.After that,their serum protein level of related molecules was detected by ELISA.Results The average concentrations of MICA,MICB,ULBP1 and ULBP2 in serum were higher in sub-health population compared with the healthy group.However,the level of ULBP3,ULBP4 and ULBP5 was lower than those in healthy group.Univariate analysis showed that only MICB has significant difference（P0.01） between the sub-healthy group and healthy group.Multivariate logistic regression analysis also confirmed this result.In addition,serum levels of MICA and ULBP3 in male was statistically significant higher than those in female（P0.05）.Additionally,ULBP2 and ULBP4 increase with age.Conclusions Together with other immune parameters,serum level of soluble MICB may be useful in the diagnosis of sub-health.