血必净对内毒素致多脏器功能障碍综合征的防治作用

Effect of XUEBIJING on Multiple Organ Dysfunction Syndrome Induced by Lipopolysaccharide in Sheep

目的探讨血必净对内毒素（LPS）致多脏器功能障碍综合征（MODS）的防治作用。方法将40只绵羊随机分为对照组（A组）、模型组（B组）、预防组（C组）和治疗组（D组），每组10只。动物全麻后，以6μg／kgLPS于30min内静脉泵入建立MODS模型。C组在静脉泵入LPS前1h、D组在泵入LPS后1h，以2ml／kg血必净（XUEBIJING）注射液静脉滴注，D组1次/d，连续3d。同时，A组静脉泵入同剂量的生理盐水。以不同时点测定各组的血气、血常规、血清生化指标的改变。结果LPS静脉泵入后，C和D组绵羊体征表现较B组有所减轻；A、B、C和D组绵羊的死亡率分别为0％、60％、40％和50％：B、C和D三组3h内氧合指数与0h和A组相比明显降低（P〈0．05或P〈0．01），但组间相比均无统计学意义（P〉0．05）；不同时点，B、C和D三组天门冬氨酸转氨酶（AST）、丙氨酸氨基转移酶（ALT）、总胆红素（TBIL）和肌酐（CRE）的含量与0h和A组相比明显升高（P〈0．05或P〈0．01）；而WBC数目3～6h则明显下降（P〈0．01），12h时开始上升，24h后骤然升高（P〈0．01）。结论血必净能改善LPS诱导的动物体征表现、降低死亡率，对肺脏、肝脏和肾脏等多脏器损伤具有一定的干预作用。

Objective To investigate the prevention and cure efficacy of XUEBIJING on multiple organ dysfunction syndrome （MODS） induced by lipopolysaccharide （LPS） in sheep. Methods Fourty sheep were randomly divided into 4 groups： the control （Group A）, model （Group B）, precaution （Group C） and cure group （Group D）, 10 sheep each group. After anesthesia, the MODS model was established by infusing LPS at 6 μg/kg within 30 minutes intravenously. Then the sheep were infused with XUEBIJING （2ml/kg） by intravenous drip before and 1 hour after injecting LPS in Group C and Group D respectively; animals in Group D received XUNBEJING （2 ml/kg） by intravenous drip once daily for 3 days. Meanwhile, the sheep were infused with isotonic Na chloride at the same dose in Group A. The indexes of blood gas, blood routine and blood biochemistry were determined at the different time points. Results After intravenous injecting with LPS, the physical sign was alleviative to some extent in Group C and Group D than that in Group B. The death rates of sheep were 0%, 60%, 40% and 50% in Group A, Group B, Group C and Group D respectively. Compared to the values of oxygenation indexes prior to injection with LPS, it decreased obviously （P〈0.05 or P〈0.01） within 3 hours in Group B, Group C and Group D, but there were not significant statistical differences observed between three groups （P〉0.05）. At the different time points, the level of aspartic transaminase （AST）, alanine aminotransferase （ALT）, total bilirubin （TBIL） and creatinine （CRE） were obviously increased compared to that prior to injection （P〈0. 05 or P〈0.01）. But the number of white blood cell （WBC） was obviously decreased at 3 h-6 h, then it began to increase at 12 h and increased significantly at 24 h （P〈 0.01）. Conclusion XUEBIJING could improve physical sign appearance, degrade the death rate of MODS induced by LPS and it had interventional effect on damage in multiple organ.