结直肠癌中MMP-7、VEGF-D和VEGFR-3的表达相关性研究

Expressions of MMP-7,VEGF-D and VEGFR-3 in colorectal carcinoma and its clinic significance

目的:通过检测结直肠癌中基质金属蛋白酶-7(Matrix metalloproteinase-7,MMP-7)、血管内皮生长因子-D(Vascularendothelial growth factor-D,VEGF-D)和血管内皮生长因子受体-3(Vascular endothelial growth factor receptor-3,VEGFR-3)的表达,探讨MMP-7与淋巴管生成因子的关系。方法:应用免疫组化法检测124例结直肠癌和70例正常黏膜组织中MMP-7、VEGF-D和VEGFR-3的表达,比较两种组织中3种蛋白表达的差异,分析其与结直肠癌临床病理指标的关系及三者的相关性。结果:MMP-7、VEGF-D和VEGFR-3表达的阳性率在结直肠癌中明显高于黏膜组织(P<0.001),MMP-7的表达与结直肠癌的肿瘤体积、Dukes分期、浸润深度及淋巴结转移有关(P<0.05);VEGF-D和VEGFR-3的表达与肿瘤体积、淋巴结转移有关(P<0.05)。结直肠癌中MMP-7和VEGF-D、VEGFR-3的表达呈正相关性(均P=0.01)。MMP-7和VEGF-D、VEGFR-3的高表达与患者的不良预后相关(P<0.05)。结论:结直肠癌中MMP-7的表达与VEGF-D、VEGFR-3的表达密切相关,可能在淋巴管新生中起协同作用,促进淋巴道转移。联合检测MMP-7、VEGF-D和VEGFR-3可能对判断结直肠癌的预后有一定的指导意义。

Objective：To study the expressions of matrix metalloproteinase-7（MMP-7）,vascular endothelial growth factor-D（VEGF-D） and（Vascular endothelial growth factor receptor-3）（VEGFR-3） in colorectal carcinomas and relationship between MMP-7 expression and lymphangiogenesis.Methods：124 primary tumors with colorectal carcinoma were studied,together with 70 normal colorectal tissues by immunohistochemistry.Results：The expressions of MMP-7,VEGF-D and VEGFR-3 were higher in the primary tumors than those in mucosa.MMP-7 expression was associated with tumor volume,Dukes stage,invasive depth and lymph node metastasis.VEGF-D and VEGFR-3 expressions were associated with tumor volume and lymph node metastasis.There was positive correlation between MMP-7,VEGF-D and VEGFR-3 in primary tumors.The higher expressions of MMP-7,VEGF-D and VEGFR-3 indicated worse prognosis in colorectal carcinoma.Conclusion：MMP-7 overexpression was closely related to the expressions of VEGF-D and VEGFR-3 that might be involved in the lymphangiogenesis.MMP-7,VEGF-D and VEGFR-3 might be helpful to predict the prognosis of colorectal carcinoma.