体质指数与大鼠急性肺损伤模型的肺损伤程度相关性研究

Correlation of body mass index and lung injury in rat models of acute lung injury

目的:急性肺损伤(Acute lung injury,ALI)发病机制复杂,死亡率高。本研究从大鼠体质指数(Body mass index,BMI)及血脂水平角度出发探讨脂质代谢是否在ALI发病机制中起着调控作用及其可能途径。方法:36只雄性SD(Sprague-dawley,SD)大鼠随机平均分为对照组(C组)、模型组(OA组)、高脂肥胖组(C-H组)、高脂肥胖模型组(O-H组),造模后预定时间点处死大鼠并取肺组织作湿重/干重比值(W/D)、肺组织病理学观察、肺组织核因子-κB(Nuclear factor-kappa B,NF-κB)表达水平,及测定炎症因子白细胞介素-1β(Interleukin-1β,IL-1β)、肿瘤坏死因子α(Tumor necrosis factorα,TNF-α)等。结果:O-H组在肺损伤病理学评分以及IL-1β、TNF-α3项指标均较OA组程度升高(P<0.05),前者肺组织NF-κBmRNA表达水平较后者明显上调,C-H组较C组相比也存在上述指标的显著差异。结论:在相同单位剂量油酸的诱导下,从mRNA及炎症因子水平提示了BMI水平与肺损伤程度呈正相关,是影响肺损伤程度的独立因子,为临床防治提供一定的参考价值。

Objective：To investigate the correlation between BMI and lung injury degree in acute lung injury of rats.Methods：A total of 60 male sprague-dawley（SD）rats were randomly divided into control group（C）,Oleic acid-treated group（OA）,control-hperlipidemia（C-H） group,and Oleic acid-hperlipidemia（O-A） group.Acute lung injury was induced by injection of OA,O-A（does,0.15 ml/kg） via intravenous vein.Rats were killed at scheduled time-points and the Lung tissues were removed for lung pathology and determination of wet/dry weight ratio（W/D）,nuclear factor-kb（NF-κB） expression,inflammatory cytokines interleukin-1β（IL-1β）,and tumor necrosis factor α（TNF-α）.Results：In O-H group,the pathology of lung injury score,IL-1β and TNF-α were significantly increased,as compared with the OA group（P0.05）.The above lung tissue expression of NF-κB mRNA in O-H group were also significantly increased than OA group.Conclusion：Under the same dose of oleic acid induction,the mRNA and the level of inflammatory cytokines suggest that BMI levels and lung injury were positively correlated with each other.