巴西莓和诺尼果粉对C57BL/6J小鼠抗8Gy剂量辐射的初步研究

The Preliminary Anti-radiation Study on C57 Mice Irradiated by 8Gy γ Ray After Gavaged Acai Based Herbal Supplementary Blend and Noni Fruit Powder

目的:比较研究巴西莓果粉Herbal Clean Energy和诺尼果粉Noni GIA通过消除自由基、降低造血细胞凋亡等作用对8Gy大剂量γ线照射后小鼠活存的影响。方法:将C57BL/6J小鼠随机分组、每组雌雄各半,单一果粉在相同灌胃剂量下采用照前灌胃10天、照后灌胃10天以及照前照后灌胃10天三种灌胃方式,首先观察了小鼠在用钴60γ线8Gy致死剂量照后40天活存率;其次在上述相同条件处理下,照后10天对小鼠外周血白细胞计数、CD4+和CD8+淋巴细胞类型、活性氧、骨髓造血细胞凋亡率等分析。结果:生理盐水灌胃组C57BL/6J小鼠受8Gy照射在第18天全部死亡(n=20,下同),死亡率100%,而照前10天灌胃后照射8Gy试验组:巴西莓和诺尼果粉组照后40天活存10/20只,诺尼果粉组第40天活存9/20,巴西莓果粉组第40天活存8/20。在照后灌胃的组别中,诺尼果粉组第40天活存7/20,巴西莓和诺尼果粉组第40天活存4/20只,巴西莓果粉组第40天活存2/20。照射前后单独或联合灌胃两种果粉组外周血白细胞均有升高,而骨髓造血细胞凋亡降低,而且果粉灌胃小鼠外周血Th/Tc比率同对照组相比明显保持于正常值范围。红细胞和血小板数据无明显变化,活性氧含量则呈现无规律表现。结论:巴西莓和诺尼果粉对小鼠抗辐射有预防作用,其细胞学表现为保持造血增殖能力、降低造血细胞凋亡,并维持Th/Tc免疫平衡;显示果粉对保持造血和免疫能力是提高抵抗辐射损伤、提高活存的基础。同时表明,服用巴西莓果粉和诺尼果粉,对人体防止辐射损伤有预防作用。

Objective： A comparative study of mice surviving under 8Gy high-dose γ ray irradiation by gavaged Acai berry based herbal clean supplementary blend and Noni fruit powder was reported.It was evaluated for eliminating free radicals,reducing the hematopoietic cell apoptosis and immuneregulation.Methods： C57BL/6 mice were randomly divided into groups of half male and half female,Acai or Noni powder used at the same dose,respectively or combinedly gavage use for 10 days before or/and after 60Co-γ irradiation at 8Gy.Firstly in the following 40 days after irradiation the surviving rate was observed;a duplicated test under the same treatments were employed for peripheral white blood cell count,CD4＋ and CD8＋ lymphocyte type,reactive oxygen species,as well as bone marrow cell apoptosis analysis.Results： The saline group C57BL/6 mice irradiated by 8Gy all died in the first 16 days（n = 20,the same in other groups）,the mortality rate 100%,while among the pre-gavaged groups,the survive rate were Acai plus Noni 10/20,Noni fruit powder group 9/20,Acai group 8/20 in the following 40 days after irradiation.And the survive rate in the groups of gavaged after irradiation were： Acai alone 2/20,Noni alone group 7/20,Acai with Noni group 4/20 in the following 40 days after irradiation（30 days after gavage）.Gavaged the fruit powder before and/or after irradiation were found all increased leukocytes in peripheral blood,and decreased bone marrow cells apoptosis,and in all the test groups the Th/Tc ratio was maintained in the normal range compared with the control group.But red blood cells and platelets without significant changes in the data,yet showed no correlation change in reactive oxygen levels.Conclusion： Acai berry and the Noni fruit powder were helpful for mouse anti-radiation and had a preventive effect,and they maintained hematopoietic cellular proliferation,decreased apoptosis under radiation,and maintained Th/Tc immune balance that were contributions to the anti-radiation.It was demonstrated that Acai berry and Noni fruit powder can improve hematopoietic ability,decrease the hematopoietic cell apoptosis,and keep immune resistance to radiation damage.It also showed that taking Herbal Clean Energy Acai berry powder and NoniGIA Noni fruit powder obviously had positive effect for preventing the radiation damage to human health.