糖-蛋白质相互作用在酶固定及蛋白质识别与分离中的应用

Applications of the Carbohydrate-Protein Interaction on Immobilization of Enzyme and on Recognition and Separation of Proteins

近些年来,糖-蛋白质相互作用的研究越来越受到研究人员的重视,它在生命过程中发挥着重要的作用,是生命体中信号传导、免疫应答、细胞黏附、病菌感染、受精、增殖、分化等许多细胞识别过程的基础。依据糖的类型不同对糖-蛋白质相互作用作了三个方面的应用总结:应用于酶的固定化,主要有壳聚糖,淀粉,琼脂糖,纤维素等多糖;应用于蛋白质的识别,主要是一些糖类芯片;应用于蛋白质的分离,主要有琼脂糖,肝素/硫酸类肝素,葡聚糖等。最后,对糖-蛋白质相互作用在生物化学领域的意义作了展望。

In recent years,carbohydrate-protein interaction has been taken attention by more and more researchers.it plays an important role in the life process and is the bases of signal transmission,immune response,cell adhesion,bacterial infection,fertilization,proliferation and differentiation in living organisms According to carbohydrate-protein interaction and the types of carbohydrates,main three different applications are as fellows： it is used in the immobilization of enzymes,basically there are chitosan,starch and agarose;it is used in recognition of proteins,in this aspect some sugar chips are mainly mentioned;it is used in separation of proteins,basically there are agarose,heparin,glucan.Finally,the applications of carbohydrate-protein interaction in biological chemistry are prospected.