二十二碳六烯酸对3T3-L1脂肪细胞脂联素mRNA的影响及其机制

EFFECTS OF DOCOSAHEXAENOIC ACID ON ADIPONECTIN mRNA AND ITS MECHANISM IN 3T3-L1 ADIPOCYTES

目的探讨二十二碳六烯酸(docosahexaenoic acid,DHA)对3T3-L1脂肪细胞脂联素表达的影响及其机制。方法不同浓度DHA处理体外培养成熟的3T3-L1脂肪细胞,并选取一定浓度DHA加与不加过氧化物酶体增殖物激活受体γ(PPARγ)拮抗剂GW-9662处理脂肪细胞,实时荧光定量PCR分析处理前后脂联素基因和PPARγmRNA表达水平的差异。结果与对照组相比,当DHA浓度为50、100 mol/L时,脂联素表达水平分别增加71.89%、106.23%(P<0.05),随着浓度的增加脂联素表达降低,当DHA浓度达到400 mol/L时,脂联素表达水平最低(P<0.05)。当DHA浓度为100 mol/L时,脂肪细胞PPARγmRNA表达增加70.24%(P<0.05)。与对照组相比,DHA中加GW-9662处理组脂联素和PPARγmRNA表达水平分别降低97.32%、90.90%(P<0.05)。结论在一定浓度范围内,DHA对脂联素表达的影响呈剂量依赖关系,推测DHA可能是通过PPARγ途径调控脂肪细胞的脂联素表达。

Objective To study the effects of docosahexaenoic acid on adiponectin mRNA and its mechanism in 3T3-l1 adipocytes.Method 3T3-L1 adipocytes were incubated with 25,50,100,200,400 μmol/L DHA,or with bull serum albumin（BSA） alone（control） for 24 h.Adipocytes were also incubated for 24 h with DHA plus GW-9662,a PPARγ antagonist,or with DHA alone.The mRNA expression of adiponectin and PPARγ was assessed by real-time fluorescence quantitative PCR.Result Both 50μmol/L and 100 μmol/L DHA increased（P0.05） the expression of adiponectin mRNA compared with the control（71.89% and 106.23% respectively）,and the lowest was in group 400μmol/L（P0.05）.The mRNA level of PPARγ in 100μmol/L group was significantly 70.24% higher as compared with the control（P0.05）.Incubating with GW-9662 and DHA,decreases in the mRNA expression of adiponectin and PPARγ were 97.32% and 90.90%,compared with the control respectively.Conclusion DHA affects the expression of adiponectin mRNA in 3T3-L1 adipocytes concentration-dependently,possibly by a mechanism involving PPARγ.[ACTA NUTRIMENTA SINICA,2012,34（2）：128-131,134]