双江古茶树与云南大叶种茶群体品种间的ISSR分析

ISSR analysis between Shuangjiang ancient tea trees and Yunnan big-leaf tea trees

采用ISSR分子标记技术对云南17份茶树样品进行了亲缘关系分析。实验结果表明,12条ISSR引物共产生193条扩增带,其中多态性条为100.0%。供试材料的遗传相似系数变异范围为0.50～0.88。通过UPGMA法,可将17份材料分为2个大类,第1大类为"红毛茶",第2大类又可分成2个亚组,其中组Ⅰ包括双江古茶树和永德大叶种,而组Ⅱ包括勐库群体品种、勐海大叶种和部分凤庆大叶种。聚类分析结果表明,双江古茶树与采自永德的茶树亲缘关系最近,而与采自勐海和凤庆大叶种的亲缘关系相对较远。

ISSR molecular markers were used to analyze the genetic polymorphism of 17 Yunnan big-leaf tea resources.The results show that 193 bands were amplified with 12 ISSR primers,and the polymorphic rate were 100.00 %.The genetic similarity of the materials varied between 0.50 and 0.88.The 17 materials were classified into 2 groups with UPGMA method,and the first group involed ＂Hongmao Tea＂,and the second one included two subgroups.The subgroupⅠincluded the Shuangjiang ancient tea trees and Yongde big-leaf,and the subgroupⅡincluded ＂Mengku＂,the Menghai big-leaf and parts of Fengqing big-leaf.The genetic relationship of the ancient tea trees from Shuangjiang and parts of Fengqing big-leaf tea was the closest,and that was more far relatively with Menghai big-leaf tea.