南京夏秋季海产品中副溶血性弧菌的污染监测及其毒力与耐药性

Surveillance on Contamination,Viroluence and Drug Resistance of Vibrio parahaemolyticus in Summer and Autumn Seafood in Nanjing

目的了解南京市夏秋季海产品中副溶血性弧菌(Vibro parahaemolyticus,VP)的污染状况以及分离菌株的毒力与药物敏感性。方法自农贸市场与饭店采取海产品样品,参考国标法(GB/T4789-2008)对样品进行检测,用科玛嘉弧菌显色培养基进行分离培养,对紫红色菌落进行PCR鉴定,对分离菌株进行神奈川试验与药物敏感试验。结果在296份样品中共检出副溶血性弧菌237株,检出率为80.1%,其中冷冻与保鲜海鱼类检出率为96.4%,贝壳类产品检出率为80.8%,鲜活鱼虾类检出率为6.7%,鲜海带类检出率为40.9%,海蜇类检出率为66.7%。VP种属特异性的基因tlh的PCR鉴定与显色培养基的鉴定结果一致;237株VP神奈川试验阳性69株;常用抗生素出现不同程度耐药,完全不耐药的分别为头孢曲松、头孢呋肟、万古霉素、美满霉素、奥复星、麦迪霉素、先锋霉素Ⅴ、丁胺卡那霉素、诺氟沙星、丙氟哌酸、复达欣等。结论南京夏秋季海产品中存在严重的副溶血性弧菌污染,分离菌株含毒力占29.1%,对16种抗生素存在不同程度的耐药。显色培养基用于海产品中副溶血性弧菌的快速检测与PCR检测结果一致。

Objective To investigate the contamination,viroluence and drug resistance of Vibrio parahaemolyticus among summer and autumn seafood in Nanjing Methods Seafood samples were collected from farm produce markets and restaurants.National standard detection methods（GB/T4789-2008） were adopted in the isolation of Vibrio parahaemolyticus by CHROMagar vibrio colored medium,and the isolated purple colonies were identified by PCR.Kanagawa test and drug sensitivity test were conducted for the isolated strains.Results Among 296 seafood samples,237 strains of Vibrio parahaemolyticus were isolated,with the detection rate of 80.1%.The detection rates of frozen and fresh-keeping marine fish,shellfish products,fresh fish and shrimps,fresh seaweed and jellyfishes were 96.4%,80.8%,6.7%,40.9% and 66.7% respectively.The detection results of species-specific tlh gene of Vibrio parahaemolyticus by PCR were in agreement with those determined by CHROMagar vibrio colored medium.Among 237 strains of V.parahaemolyticus,Kanagawa tests showed positive results in 69 strains.Drug sensitivity tests showed that V.parahaemolyticus exhibited various degrees of resistance to the common antibiotics,but it was highly sensitive to ceftriaxone,cefuroxime,vancomycin,minocycline,ofloxacin,midecamycin,cephalosporin V,amikacin,norfloxacin,ciprofloxacin and ceftazidime.Conclusions There exists serious contamination by V.parahaemolyticus in seafood produced in summer and autumn in Nanjing.29.1% of the isolated strains are virulent and some strains are resistant to 16 kinds of antibiotics at different degrees.The results of rapid detection of V.parahaemolyticus in marine products by CHROMagar vibrio colored medium are consistent with those by PCR.