乳腺癌与载脂蛋白B基因多态性关系

Association of apolipoprotein B gene polymorphism with breast cancer

目的探讨载脂蛋白B（ApoB）基因-516位基因多态性与女性乳腺癌发生的关系。方法运用聚合酶链反应-限制性片段长度多态性（PcR—RFLP）方法检测200例乳腺癌患者（实验组）和200例正常女性（对照组）的ApoB基因-516位C／T基因型和等位基因的分布，并进行对比分析及相关性研究。结果乳腺癌患者中CC、CT基因型的频率分别是78．00％、22．00％，而对照组中则分别为91．50％、8．50％；乳腺癌患者中C、T等位基因的频率分别为89．00％、11．00％，对照组中则分别为95．75％、4．25％，差异均有统计学意义（P〈0．05）。CT型个体罹患乳腺癌的风险显著高于CC型个体（P〈0．05）。调整年龄、体质量指数后，携带cT型的个体较cc型罹患乳腺癌的风险增加（P〈0．05）。结论ApoB基因．516位基因多态性与乳腺癌的易感性相关。

Objective To investigate the association of the apolipoprotein B （ApoB） gene-516 C/T polymorphisms with breast cancer in Chinese female population. Methods ApoB-516C/T polymorphisms was genotyped by polymerase chain reaction （PCR） and restrictiori fragment=length polymorphism （RFLP） in 200 breast cancer patients and 200 controls. The contrastive analysis and the relevant analysis of geno-type and allele distribution was performed. Results The frequency of CC and CT genotype in breast cancer group and control group was 78.00% , 22. 00% , and 91.50% , 8.50% , respectively. The frequency of C and T allele in breast cancer group and contro） group was 89. 00%, 11.00%, and 95. 75%, 4. 25%, respectively. The risk of breast cancer in the individuals with 516C/T polymorphism was significantly increased. After adjusted for age, and body mass index （BMI） by multiple logistic regression analysis, the odds ratio of T allele carriers for breast cancer was increased as compared with that of non carriers （ OR = 3. 036 ; 95% CI = 1. 668-5. 527 ）. Conclusion The ApoB gene-516C/T polymorphism is associated with breast cancer in Chinese female population.