转化生长因子-β1及p21对乳腺癌干细胞的作用

The research of the transforming growth factor β1 and p21 effect on breast cancer stem cells

目的观察转化生长因子-β1（TGF-β1）诱导乳腺癌耐阿霉素细胞株MCFT／ADM中乳腺癌干细胞富集的作用，比较乳腺癌干细胞和MCF7／ADM中p21基因和蛋白水平的表达差异。方法流式细胞仪检测阿霉素耐药乳腺癌细胞（ADM细胞）、乳腺细胞球培养法富集的乳腺癌细胞（sc细胞）及加入TGF-β1细胞因子诱导的乳腺癌细胞（sc—T细胞）中乙醛脱氢酶1（ALDH1）阳性细胞比例；比较上述3组细胞微球形成能力差异；逆转录-聚合酶链反应（RT—PCR）及Westernblot检测3组细胞中p21基因及蛋白水平的表达差异。结果ADM组、SC组及Sc—T组中ALDH1阳性细胞比例分别为8．49％、14．94％及23．44％；细胞微球形成率分别为5．29％、11．67％、23．13％；3组细胞中p21mRNA及蛋白水平的表达均逐渐升高。结论TGF-β1能诱导乳腺癌干细胞的富集并使其具有更强的自我更新能力。P21在乳腺癌干细胞中高表达。

Objective To detect the enrichment of breast cancer stem cells induced by transfor- ming growth factor 131 （TGF-β1） , and the difference of p21 expression between breast cancer stem cells and MCFT/ADM cells. Methods Flow cytometry detect the proportion of aldehyde dehydrogenase 1 （ALDH1） positive cells in MCF7/ADM, breast cancer stem cells enriched by mammosphere and breast cancer stem cells induced by TGF-β1. To detect the mammoshpere formation ability in above three teams by mammoshpere formation test. To detect the p21 expression in three teams above by reverse transcription- polymerase chain reaction （RT-PCR） and Western blotting. Results The proportion of ALDH1 positive cells in the three teams were 8.49% , 14. 94% , 23.44% respectively. The mammosphere formation rate were 5.29% , 11.67%, 23. 13% respectively. The expression of p21 increased in the three teams. Conclusion TGF-β1 can induce the enrichment of breast cancer stem cells, and enhance it＇ s self-renewal ability. The expression of p21 is higher in breast cancer stem cells than common breast cancer cells.