乳腺癌易感基因1、Ki-67在青年乳腺癌患者组织中的表达及其临床意义

The clinical significance of breast cancer susceptibility gene 1 and Ki-67 in young breast cancer tissues

目的探讨乳腺癌易感基因1（BRCA1）和Ki-67蛋白在青年乳腺癌患者组织中的表达及临床意义。方法选取年龄≤35岁青年乳腺癌89例，对照组为乳腺纤维腺瘤35例及年龄〉35岁的乳腺癌85例，运用免疫组织化学法检测Ki-67和BRCA1蛋白的表达，并结合临床病理因素进行相关分析。结果在维吾尔族青年乳腺癌患者中，BRCA1的表达（39．33％）低于乳腺纤维腺瘤（74．29％）及年龄〉35岁组（58．82％，P〈0．05），Ki-67的表达（71．91％）高于乳腺纤维腺瘤（28．57％）及年龄〉35岁组（57．64％，P〈0．05）；青年乳腺癌患者的病理组织学分级、淋巴结转移、原癌基因表皮生长因子受体2（Her-2）表达与BRCA1及Ki-67表达相关（P〈0．05）；青年乳腺癌患者Ki-67与BRCA1蛋白的表达呈负相关（r=-0．265，P〈0．05）。结论青年乳腺癌中BRCA1和Ki-67蛋白表达的异常及BRCA1的失表达，可能与发病早、恶性度高有关。

Objective To study the expression of breast cancer ansceptibility gene 1 （ BRCA1 ） and Ki-67 in youth patients with breast cancer and the clinical significance. Methods There were 89 Uignr patients under 35 years with breast cancer in experimental group, and the control group consisted of 85 patients older than 35 year-old women with breast cancer and 35 cases of benign mammary fibroadenoma. Immunohistochemistry was used to detect the expression of BRCA1 and Ki-67 proteins and clinical pathological features were analyzed. Results The expression of BRCA1 protein in youth patients with breast cancer was lower than in patients with mammary fibroma or older breast cancer patients, and expression rate was 39. 33%, 74. 29%, and 58.82% separately. The expression of Ki-67 protein in youth patients with breast cancer was higher than in patients with mammary fibroma or 35 years older breast cancer patients, and ex- pression rate was 71.91%, 28. 57%, and 57.64% separately. The positive expression of BRCA1 and Ki-67 was correlated with the pathological grade, lymph node metastasis and expression of human epidermalgrowth factor receptor-2 （Her-2）. The expression of BRCA1 protein was negatively correlated with Ki-67 expression in youth patients with breast cancer. Conclusion The aberrant expression of BRCA1 and Ki-67 protein and negative expression of BRCA1 protein may be related to early onset and high malignant degree in youth patients with breast cancer.