免疫结核球蛋白、血管内皮生长因子-C在肝癌组织中的表达及意义

The expression and significance of the correlation factor of unfolded protein response： immunoglob-ulin-binding protein, vascular endothelial growth factor-C in hepatoma

目的检测免疫结核球蛋白（Bip）、血管内皮生长因子（VEGF）-C在肝癌组织中的表达，探讨其在肝癌生长中的作用机制。方法采用逆转录-聚合酶链反应（RT—PCR）检测术中所取新鲜的42例肝癌标本、15例同个体癌旁1．0cm肝组织和15例正常肝组织中Bip、VEGF—C的mRNA表达，应用Westernblot方法验证57例肝癌组织和正常肝组织标本中Bip及VEGF．C在蛋白水平的表达。结果肝癌组织、癌旁肝组织、正常肝组织中BipmRNA相对表达量分别为0．4772±0．0401、0．4332±0．0488、0．4301±0．0398，BipmRNA在3组之间的表达呈下降趋势（P〈0．05）。肝癌组织、癌旁肝组织、正常肝组织中VEGF．CmRNA相对表达量分别为0．4447±0．0335、0．4195±0．0334、0．4019±0．0259，VEGF—CmRNA在3组之间的表达呈下降趋势（P〈0．05）；实验对肝癌组织和正常肝组织中Bip和VEGF—C的蛋白表达进行定性验证：在肝癌组织和正常肝组织中均有两种蛋白的表达，但肝癌组织中的表达明显高于正常肝组织。结论在肝癌的生长过程中存在未折叠蛋白反应（UPR）的激活。在肝癌组织中Bip、VEGF—CmRNA和蛋白的表达一致，均呈高表达。这些基因和蛋白表达的改变可能与肝癌的发生、生长及转移密切相关。

Objective To detect the express of immunoglobulin-binding protein （Bip）, vascular endothelial growth factor （VEGF） -C in the tissue of hepatoma; demonstrating that it exist activation of un- fold protein response （UPR） in process of growth, and the expression of UPR was higher than normal tis- sue ; approaching the mechanism of action in the growth of hepatoma, searching the new target for the thera- py of hepatoma. Methods To study the mRNA of Bip, VEGF-C in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse transcription-pol- ymerase chain reaction （RT-PCR）. To qualitation check the expression of Bip and VEGF-C in the level of protein by Western blotting. Results The express of Bip mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4772±0. 0401, 0. 4332±0. 0488, 0. 4301±0. 0398;the express of Bip mRNA was higher in hepatoma than others （P 〈 0. 05 ）. The express of VEGF-C mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4447±0. 0335, 0. 4195±0. 0334, 0. 4019±0. 0259. The express of VEGF-C mRNA was higher in hepatoma than others （P 〈 0. 05 ）. The experiment indicate that the two protein all express in the hepatoma tissue and normal hepatic tissue, but they were higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist activation of UPR in process of growth. The express of Bip, VEGF-C protern and their mRNA was accordant, all of them were higher in hepatoma tissue than in normal hepatic tissue. The changes of these gene and protern is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma.