Livin与Caspase-9在皮肤鳞状细胞癌组织中的表达及其临床意义

Expression and clinical significance of Livin and Caspase-9 in skin squamous cell carcinoma tissue

目的检测Livin和Caspase-9蛋白在皮肤鳞状细胞癌（SCC）中的表达，并探讨其临床意义。方法应用免疫组织化学SP法检测56例SCC组织、20例正常皮肤组织中Livin和Caspase-9蛋白的表达。结果SCC组织中Livin蛋白的阳性表达率（73．2％）高于正常皮肤组织（0．0％），差异有统计学意义（P〈0．05）；Livin蛋白的表达与肿瘤的分化程度显著相关，其阳性率随SCC病理分级的降低而升高，差异有统计学意义（P〈0．05）；在有淋巴结转移的SCC组织中的阳性率（93．3％）明显高于无淋巴结转移者（65．9％），差异有统计学意义（P〈0．05）。SCC组织中Caspase-9蛋白的阳性表达率（30．4％）低于正常皮肤组织（90．0％），差异有统计学意义（P〈0．05）；在SCC中Caspase-9蛋白的表达与肿瘤的分化程度显著相关（P〈0．05），即随SCC病理分级的增高而升高；有淋巴结转移的SCC组织中Caspase-9蛋白的阳性表达率（6．7％）低于无淋巴结转移者（39．0％），差异有统计学意义（P〈0．05）。两种蛋白在SCC组织中的表达呈负相关（rs=0．612，P〈0．05）。结论Livin在SCC组织中高表达，Caspase-9在SCC组织中低表达，且与Livin的表达呈负相关，提示两者可能共同参与SCC发病过程。

Objective To investigate the expression and the clinical significance of Livin and Caspase-9 protein in squamous cell carcinoma tissue. Methods Livin and Caspase-9 protein expression was detected in 56 SCC and 20 normal skin specimens by SP immunohistochemical technique. Results The positive expression rate of Livin protein in SCC （73.2%） was higher than that in normal lung tissue （0. 0% ）. There was significant difference in the positive expression rate of Livin between squamous cell carcinoma and normal skin tissues （ P 〈 0. 05 ）. The expression of Livin protein was correlated with the dif- ferentiation of squamous cell carcinoma and the positive expression rate of Livin protein was increased in squamous cell carcinoma from high differentiation to poor differentiation with a statistically significant differ- ence （ P 〈 0. 05 ）. The positive expression rate of Livin protein in SCC tissue was related with lymph node metastasis （P 〈 0.05 ）. Caspase-9 protein showed lower expression in SCC （30. 4% ） than in nonnallung tissue （90. 0% ） （P 〈0. 05）. The expression of Caspase-9 is correlated with the differentiation of squa- mous cell carcinoma. The positive expression rate of Caspase-9 was decreased in squamous cell carcinoma from high differentiation to poor differentiation with a statistically significant difference （ P 〈0. 05 ）. The positive expression rate of Caspase-9 protein in SCC tissue was related with lymph node metastasis （ P 〈 0. 05）. The expression of Livin protein was negatively related to that of Caspase-9 protein in SCC tissues （rs = -0. 612,P 〈 0. 05 ）. Conclusion The expression of Livin protein in SCC was high, while the posi- tive expression of Caspase-9 protein in SCC was lower, and there was a negative correlation between them, and both Livin and Caspase-9 may play important roles in the carcinogenesis of SCC together, which may provide the new ideas for the clinical diagnosis, treatment and prognosis judgement of SCC.