芦笋老茎堆肥中高温纤维素分解菌的分离、筛选及鉴定

Isolation,Screening and Identification of Thermophilic Cellulolytic Bacteria in Old Stalk of Asparagus Compost

采用纤维素刚果红培养基在45℃下从芦笋老茎堆肥中分离筛选出52株高温纤维素分解菌。通过水解圈直径与菌落直径比值的比较,进一步获得了6株纤维素分解能力较强的菌株。经菌落形态观察、革兰氏染色、芽孢染色和生理生化试验,6株高温纤维素分解菌初步鉴定为需氧芽孢杆菌(Bacillus spp.)。

In this experiment, 52 strains of thermophilic cellulolytic bacteria were isolated and screened from the old stalk of asparagus compost using cellulose - congo red culture medium at 45 ~C. By comparing the ratio of hydrolytic circle diameter to colony diameter, 6 strains of eellulolytic bacteria with higher efficient were selected, and they were identified as Bacillus spp. through morphological observation, Gram＇ s staining, spore staining and physiological- biochemical experiments.