人β防御素2在子宫腺肌症患者在位和异位内膜组织中的表达

Expressions of human β defensin-2 in eutopic and ectopic endometrium of women with adenomyosis

目的检测人β防御素2（hBD-2）在正常子宫内膜和子宫腺肌症（AM）患者的在位和异位内膜组织中的基因和蛋白表达。方法选择AM和非子宫内膜异位症（EMS）的子宫肌瘤患者各25例，分别取AM异位病灶组织（AM异位内膜组）和相应的在位子宫内膜组织（AM在位内膜组）以及子宫肌瘤患者的子宫内膜组织（对照组）。采用实时荧光定量PCR检测各组内膜组织中hBD-2和白细胞介素-1β（IL-1β）、白细胞介素石（IL-6）、肿瘤坏死因子α（TNF—α）等的基因表达及其相关性，并免疫组化检测其蛋白的表达。结果将各组的hBD-2、IL-18、IL-6和TNF-α的基因表达进行比较，AM异位内膜组与AM在位内膜组、AM在位内膜组与对照组之间差异均无统计学意义（P均〉0．05）。AM异位内膜组hBD-2和IL-1β的基因表达分别为0．0320（0．0095～0．0690）和0．0427（0．0038～0．0975），对照组分别为0．0034（0．0025～0．0424）和0．0080（0．0040～0．0251），前者均明显高于后者（P值分别为0．032和0．025）。AM异位内膜组hBD-2的基因表达与该组的TNF—α的基因表达呈正相关（r=0．857，P=0．014），与IL-1β、IL-6的基因表达无相关性（r值分别为0．750、0．464，P值分别为0．052、0．464）。结论AM的形成可能与异位病灶组织中的hBD-2的表达无明显关系，这可能与AM患者异位病灶组织中炎症因子无明显上调有关。

Objective To investigate the expression of human β defensin-2（hβD-2）in the eutopic and eetopic endometrial tissue in patients with adenomyosis and in women with normal endometrium. Methods Twenty-five hysteromyoma patients with adenomyosis （AM）and 25 hysteromyoma patients without endometriosis （EMS） were selected and divided into three groups：AM ectopic endometrium group, AM eutopic endometrium group and control group（ endometrial tissue in patients with hysteromyoma）. The level of mRNA expressions of hβD-2, interleukin-1β （ IL-1β ）, intedeukin-6 （ IL-6 ） and tumor necrosis factor-α （ TNF-α ） was investigated quantitatively using Real Time PCR （RT-PCR） and the corresponding protein level was detected by immunohistochemistry. Results Comparing the expression of hβD-2, IL-1β, IL-6,TNF-α genes among the three groups, there was no significant difference between ectopic endometrium group and eutopic endometrium group and there was also no significant difference between eutopic endometrium group and the control group. （ P 〉 0.05 ）. The expression of hβD-2 and IL-1β were 0. 0320 （ 0. 0095 - 0. 0690 ） and 0. 0427 （ 0. 0038 - 0. 0975 ） in the ectopic endometrium group, and they were 0. 0034 （0. 0025 -0. 0424）and 0. 0080 （0. 0040 - 0. 0251 ）in the control group,respectively. They were both significantly higher in ectopic endometrium group than in the control group （P 〈 0. 05 ）. In the ectopic endometrium group hβD-2 expression was positively correlated with the level of TNF-α（ r = 0. 857, P = 0. 014 ）, and it had no correlation with both IL-1β and IL-6 （ r = 0. 750, P = 0. 052 ; r = 0. 464,P = 0. 464; respectively）Conclusion HβD-2 might not play an important role in the formation of adenomyosis. It may be related to no significant up-regnlation of inflammatory factors in ectopie lesion tissue.